J Oral Pathol Med   PMID: 24646032 (2014)

RECK overexpression reduces invasive ability in ameloblastoma cells.

Liang QX1, Liang YC, Xu ZY, Chen WL, Xie HL, Zhang B.


摘要:

BACKGROUND:

Ameloblastoma is a frequent odontogenic neoplasm characterized by local invasiveness and high risk of recurrence. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a tumor suppressor that inhibits metastasis and angiogenesis. The aim of this study was to investigate effects of RECK overexpression on invasive potential in ameloblastoma cells.

METHODS:

Lentiviral vectors containing human RECK gene were created and subsequently stably transfected into immortalized ameloblastoma cell line hTERT+ -AM. Functional characteristics of hTERT+ -AM cells with stable RECK overexpression included proliferation, migration, invasion, and regulation of matrix metalloproteinases (MMP)-2, MMP-9 measured by zymography or commercially available assays.

RESULTS:

The stable and higher expression of RECK mRNA and protein (P < 0.01) was detected in RECK-transfected hTERT+ -AM cells. RECKoverexpression caused a decrease in migration and invasion (P < 0.01) for hTERT+ -AM cells and a decrease in activity of MMP-2, MMP-9 (P < 0.01). Proliferation was not affected by RECK overexpression (P > 0.05).

CONCLUSIONS:

Overexpression of RECK gene significantly inhibited cell invasive ability of hTERT+ -AM cells, suggesting RECK may be a new target for ameloblastoma treatment.

© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

KEYWORDS:

ameloblastoma; invasion; matrix metalloproteinase; reversion-inducing cysteine-rich protein with Kazal motifs

下载 PDF 文件
  • 侧边栏广告 - 模式动物成功案例
  • 侧边栏广告 - 科研奖励基金计划
  • 侧边栏广告-积分兑换礼品