Biomed Pharmacother 66:57 (2012)
The Protective Effect of 17β-estradiol Against Hydrogen Peroxide-nduced Apoptosis on Mesenchymal Stem Cell.
Chen HY, Zhang X, Chen SF, Zhang YX, Liu YH, Ma LL, Wang LX.
Central Laboratory for Experimental Medicine, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, Liaocheng, 252000, China.
This study was designed to investigate the function of 17β-estradiol (17β-E2) against oxidative stress on the cell death of mice bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H(2)O(2)). BMSCs were treated with 17β-E2 for 24h and then treated with 100μM H(2)O(2) for 1h. Cell viability, apoptosis, caspase-9 mRNA, JNKs (Jun N-terminal kinases) and c-Jun protein expression in BMSCs were evaluated. Cell apoptosis of BMSCs were increased in a dose-dependent manner after treated with H(2)O(2) compared to control group. But pretreatment with 17β-E2 can inhibit apoptosis of BMSCs, preserve the mitochondrial transmembrane potential, decrease caspase-9 mRNA, JNK1/2 and c-Jun protein expression. In conclusion, 17β-E(2) exerts antiapoptotic effects in BMSCs which related to the mitochondria death pathway and JNKs pathway. The study revealed that 17β-E(2) can reduce the donor stem cells damage.