小鼠β防御素3 (BD-3) (Murine)

BD-3(Murine) 小鼠β防御素3 (BD-3) (Murine) MEOPP-0203
产品货号: MEOPP-0203
产品规格:
价格:


产品描述:
Beta defensin-3, also known as BD-3 and DEFB-3, is a membrane active cationic peptide that functions in inflammation and innate immune responses and coded by Defb 3 gene on chromosome 8 in mouse. There are at least 30 β-defensins which are distinguished from α-defensins by the connectivity pattern of their three intramolecular disulfide bonds. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 productions and inhibits TIMP1 and 2 expressions.


表达系统 :
Escherichia coli


溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.


产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.


存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.


分子量:
Approximately 4.6 kDa, a single non-glycosylated polypeptide chain containing 41 amino acid residues.


内毒素:
Less than 1 EU/μg of BD-3 as determined by LAL method.


产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.


生物学活性:
The ED50 determined by anti-microbial activity against E.coli. is less than 20 μg/mL, corresponding to a specific activity of > 50 IU/mg.


产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.


氨基酸序列:
KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K


产品纯度:
> 95% by SDS-PAGE and HPLC analyses.