人成纤维细胞生长因子19(FGF-19) (Human)

FGF-19(Human) 人成纤维细胞生长因子19(FGF-19) (Human) HEGFP-0619
产品货号: HEGFP-0619
产品规格:
价格:


产品描述:
Fibroblast growth factor-19 (FGF19) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4 exist. Unlike most FGFs which bind to and activate more than one FGF receptor, FGF19 is a specific ligand for FGF R4.


表达系统 :
Escherichia coli


溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.


产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.


存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.


分子量:
Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.


内毒素:
Less than 1 EU/μg of FGF-19 as determined by LAL method.


产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.


生物学活性:
The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/mL, corresponding to a specific activity of > 6.7 × 103 IU/mg.


产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.


氨基酸序列:
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK


产品纯度:
> 95% by SDS-PAGE and HPLC analyses.