人白细胞介素4 (IL-4) (Human)

IL-4(Human) 人白细胞介素4 (IL-4) (Human) HEILP-0401c
产品货号: HEILP-0401c
产品规格:
价格:
Cyagen团队特别针对免疫细胞、干细胞培养开发的一系列CTRSTM高活性细胞因子产品,产品全380余种,囊括所有细胞因子家族,种属齐包括人、鼠、其他种属,拥有高活性、高纯度、高稳定性、无热源、无外源因子污染等特点,更适合干细胞、再生医学、免疫学等研究使用。

产品描述:
Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.


表达系统 :
Escherichia coli


溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.


产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.


存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.


分子量:
Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.


内毒素:
Less than 1 EU/μg of IL-4 as determined by LAL method.


产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.


生物学活性:
The ED50 determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg.


产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.


氨基酸序列:
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS


产品纯度:
> 98% by SDS-PAGE and HPLC analyses.