牛碱性成纤维细胞生长因子(bFGF) (Bovine)

产品货号: BEGFP-0601
产品规格:
价格:


产品描述:
Bovine bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Bovine bFGF shares 95% amino acid sequence identity with murine bFGF, and 97% amino acid sequence identity with rat. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. It is also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.


表达系统 :
Escherichia coli


溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.


产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.


存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.


分子量:
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.


内毒素:
Less than 1 EU/μg of bFGF as determined by LAL method.


产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.


生物学活性:
The ED50 determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg.


产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.


氨基酸序列:
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS


产品纯度:
> 97% by SDS-PAGE and HPLC analyses.