人生长调节致癌基因α/黑色素瘤生长刺激因子(GRO-α/MGSA/CXCL1) (Human)

GRO-α/MGSA/CXCL1(Human) 人生长调节致癌基因α/黑色素瘤生长刺激因子(GRO-α/MGSA/CXCL1) (Human) HECXP-0101
产品货号: HECXP-0101
产品规格:
价格:


产品描述:
The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.


表达系统 :
Escherichia coli


溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.


产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.


存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.


分子量:
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.


内毒素:
Less than 1 EU/μg of GRO-alpha/CXCL1 as determined by LAL method.


产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.


生物学活性:
The biologically active determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-50 ng/mL.


产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.


氨基酸序列:
ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN


产品纯度:
> 97% by SDS-PAGE and HPLC analyses.