Animal Models and VectorBuilder-Related Seminar 26: University of California, Irvine

Date: Apr 12, 2017


CRISPR/Cas9 and TurboKnockout: Technologies for Rapid Generation of Knockout/Knockin Mouse Models

While genetically engineered mouse/rat models have been used widely in biomedical research, the design and generation of these models can be complicated, time-consuming and costly. In this presentation, Dr. Heighington will briefly review the traditional pronuclear injection based transgenics and ES cell homologous recombination based gene targeting, and go over the recently developed CRISPR-mediated genome editing technology, with an emphasis on the pros and cons of each technology and their applications. She will then introduce TurboKnockout, the newest ES-cell based gene targeting technology which allows the generation of conditional knockout/knockin mouse models in as fast as 6 months, 4-6 months shorter than the standard approach. Lastly, Dr. Heighington will highlight a novel online tool called VectorBuilder, which promises to revolutionize the way DNA vector cloning is done.


Speaker: Dr Cassandra Heighington, Territory Manager of Cyagen Biosciences

Date: Wednesday, April 12, 2017

Time: 12:00-1:00 PM

Location: Bio Sci 3, room 2120
                University of California, Irvine
                Irvine, CA 92697-1075, USA