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Embryoid Body (EB) Formation Medium

Embryoid Body (EB) Formation Medium MUXES-90051
Catalog Number:
MUXES-90051
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OriCellTM Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of EB. The medium can be used to form EB from hanging drops in suspension culture on non-adhesive petri dishes.

Description:
Embryonic stem cells (ESCs), derived from the inner cell mass (ICM) of blastocyst-stage embryos, are pluripotent and have a virtually unlimited capacity of self-renewal. They can differentiate into all cell types comprised of all three embryonic germ layers (ectoderm, mesoderm and endoderm). The formation of embryoid bodies (EBs) is a principal step in the differentiation of ESCs.  When maintained in the EB formation medium and in the absence of MEF feeder layers, ES cells differentiate spontaneously and form three-dimensional aggregates. This structure facilitates multicellular interactions in which cell-cell contact occurs and gap junctions are established.

Highlights:
- Highly effective in inducing human and mouse embryonic stem cells to form embryoid bodies (EB).
- Batch-to-batch consistency.

Kit Components:

 Embryoid Body (EB) Formation Basal
 Medium
 435 mL
 Embryoid Body (EB) Formation-
  Qualified Fetal Bovine Serum
 50 mL
 Penicillin-Streptomycin  5 mL
 Glutamine  5 mL
 Non-Essential Amino Acid  5 mL
 2-Mercaptoethanol  500 μl

Stability/Storage:
- All products should be stored in the dark.

- Embryoid Body Formation Basal Medium is stable at 2-8°C for up to one year; other components are stable at -20°C for up to two years.
- Products should be discarded beyond the labeled expiration date.
- Once prepared, the fully supplemented (complete) medium can be stored for up to one month when stored in the dark at 2-8°C.
- For optimal performance, repeated warm-cooling and freeze-thawing should be avoided.

Quality Control:
Tested for perfomance on EB formation. The standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
3. Osmolality
4. Endotoxin

Intended Use:
Intended for non-human laboratory research use only.