What is a knockout rat and why are they important for research?

A knockout rat is a genetically engineered model where specific genes are intentionally deactivated. These rats are essential for studying gene functions and their effects on various health conditions, making them invaluable for advancing human genetics research and medical treatment development.

Custom Knockout Rat Models

Knockout Rats

Explore Cyagen's knockout rat services, utilizing advanced Targeted Gene Editing technology for precise and efficient genome editing. Our knockout rats are vital for studying human diseases and advancing drug development, ensuring impactful research outcomes.

Precision Engineering

Count on precise genetic alterations for reliable results.

Diverse Strain Availability

Access strains such as SD, Wistar, LE, F344, Norway, Lewis, and more.

Guaranteed Results

Get animals or your money back.

Overview

Workflow

FAQs

Overview

Precision Knockout Rat Models

Cyagen's knockout rat models leverage the cutting-edge Targeted Gene Editing technology to achieve targeted gene inactivation, addressing challenges previously faced in rat genetic studies. These models are crafted to generate precise frameshift mutations or targeted deletions of critical gene domains, facilitating advanced disease modeling and drug discovery. By targeting specific genomic sites, our technology minimizes off-target effects and ensures the production of rats with constitutive gene knockouts. This makes our rats highly effective for studying complex human diseases, providing researchers with reliable tools to explore genetic functions and validate therapeutic targets.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

What Services Are Offered for Knockout Rats

Targeted Gene Editing

AI-enhanced HDR technology for precise in vivo genome engineering

Rodent Breeding

Scalable colony expansion with full genotyping support

Cryopreservation & Recovery

Preserve and revive rodent strains on demand

Rodent Phenotyping

Full-spectrum analysis for rodents model

Neuroscience CRO Platform

Validated CNS models with behavioral and molecular efficacy data

Cardiometabolic CRO Platform

IND-ready studies using genetic and induced disease models

Workflow

Workflow and Delivery

Navigate our comprehensive workflow designed to efficiently generate knockout rats, leveraging the precision of Targeted Gene Editing technology to meet specific research demands. Our process ensures timely progression from gene strategy design to the delivery of founder rats, facilitating advancements in genetic research.

Targeted Gene Editing Technology

Leverage our cutting-edge Targeted Gene Editing technology for rapid and precise knockout rat generation, tailored to meet stringent research specifications.

Stage	Description	Turnaround Time
Knockout Strategy Design	Collaborate to design a nuclease-mediated strategy tailored to your specific gene of interest, maximizing on-target activity while minimizing off-target effects.	1-4 days
Nuclease Expression Vector Construction	Construct the knockout vector based on client-approved strategy. Where needed, efficacy tests in cell culture can be conducted.	2-3 weeks
Nuclease Injection into Rat Eggs	Co-inject ribonucleoprotein (RNP) into fertilized rat eggs, and implant to obtain founders.	SD: 8-10 weeks, Long Evans: 10-16 weeks
Founder Screening	Screen pups by PCR to identify those with successful knockout modifications. Where needed, Sanger sequencing can be conducted.	1-2 weeks
Breeding Founders to Obtain F1	Breed the founders to wildtype rats and genotype their offspring to ensure transmission of the knockout allele.	12-16 weeks

Note:
Turnaround times do not include periods required for institutional approval or shipping. Our workflow can adapt to various strains as per request. Pricing details and further information are available upon inquiry.

FAQs

Citation Database

Molecular Therapy: Methods & Clinical Development, March, 2025

Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing

【Other】

Gut, February, 2025

E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression

【Other】

Cell Death & Disease, February, 2025

Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage

【Other】

Molecular Therapy, February, 2025

Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer

【Other】

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