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Knockin Rats
Cyagen's knockin rats provide targeted genetic alterations to meet complex research demands, encompassing everything from point mutations to large-fragment knockins. These rats are invaluable for modeling human diseases and studying gene function, offering robust, study-ready cohorts that accelerate the pace of preclinical research.
Precision Engineering
Count on precise genetic alterations for reliable results.
Diverse Strain Availability
Access strains such as SD, Wistar, LE, F344, Norway, Lewis, and more.
Guaranteed Results
Get animals or your money back.
Overview
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Overview
Advanced Knockin Rat Models
At Cyagen, we harness the precision of targeted gene editing technology to create knockin rats that offer unparalleled accuracy in genetic modifications. Our services extend from simple point mutations that simulate human genetic disorders to the insertion of reporter genes like EGFP or mCherry at specific loci. These modifications enable researchers to track gene expression dynamically or replace endogenous rat genes, providing insights into gene functionality and disease mechanisms.

Trust Cyagen for your advanced research needs in gene function and disease modeling. Our knockin rat models are specifically designed to facilitate sophisticated genetic studies, offering modifications up to 20kb with guaranteed fidelity and reproducibility.
Explore Ready-to-Use Mouse Models
Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.
What Services Are Offered for Knockin Rats
Targeted Gene Editing
AI-enhanced HDR technology for precise in vivo genome engineering
Rodent Breeding
Scalable colony expansion with full genotyping support
Cryopreservation & Recovery
Preserve and revive rodent strains on demand
Rodent Phenotyping
Full-spectrum analysis for rodents model
Neuroscience CRO Platform
Validated CNS models with behavioral and molecular efficacy data
Cardiometabolic CRO Platform
IND-ready studies using genetic and induced disease models
Workflow
Workflow and Delivery
Our refined workflow ensures the precise and timely creation of knockin mouse models tailored to your research specifications. Utilize our advanced TurboKnockout® and Targeted Gene Editing technologies to achieve unparalleled accuracy and efficiency in gene targeting, facilitating faster transitions from design to delivery.
Targeted Gene Editing Technology
Any Locus
ROSA26/H11
Point Mutation
Targeted Gene Editing Technology for Knockin Rats at Any Locus
Targeted Gene Editing Technology for Knockin Mice at Any Locus
Explore the versatility of Cyagen’s Targeted Gene Editing technology for creating large-fragment and conditional knockin rat models. This advanced method allows for precise genetic modifications at any locus, making it ideal for broad applications, including disease modeling and functional genomics.
Stage Description Turnaround Time
Strategy Design Develop a nuclease-mediated strategy tailored to your gene of interest, including gRNA and donor vector design. 1-4 days
Vector Construction Construct the knockin vector based on client-approved strategy. Where needed, efficacy tests in cell culture can be conducted. 6-8 weeks
Targeted Gene Editing Injection Co-inject ribonucleoprotein (RNP) and the donor vector into fertilized mouse eggs, and implant to obtain founders. SD: 8-12 weeks, Long Evans: 10-16 weeks
Founder Screening Screen pups by PCR to identify those with successful knockin or floxed modifications. Where needed, Sanger sequencing can be conducted. 2-4 weeks
Breeding Founders Breed the founders to wildtype rats and genotype their offspring to ensure transmission of the knockin allele. 12-16 weeks
Southern Blotting (Optional) Confirm integration and copy number of the knockin fragment in founders or F1 progeny. 2-4 weeks
Note:
  • Standard services facilitate modifications up to 2kb. Projects requiring larger fragments may involve additional costs and extended timelines.
  • We primarily use SD and Long Evans rat strains, with options for customization.
Targeted Gene EditingTechnology for Knockin Rats at ROSA26/H11 Locus
Targeted Gene EditingTechnology for Knockin Rats at ROSA26/H11 Locus
Delve into our specialized Targeted Gene Editing technology optimized for targeting the ROSA26/H11 locus in rats. Known for its reliability as a safe harbor site, this method ensures consistent and stable expression of transgenes without disrupting endogenous gene function.
Stage Description Turnaround Time
Strategy Design Develop a nuclease-mediated strategy tailored to your gene of interest, including gRNA and donor vector design. 1-4 days
Vector Construction Construct the ROSA26/H11 knockin vector based on client-approved strategy. Where needed, efficacy tests in cell culture can be conducted. 4-6 weeks
Targeted Gene Editing Injection Co-inject ribonucleoprotein (RNP) and the donor vector into fertilized mouse eggs at the ROSA26/H11 locus, followed by embryo implantation to obtain founders. SD: 8-12 weeks, Long Evans: 10-16 weeks
Founder Screening Screen pups by PCR to identify those with successful knockin at ROSA26/H11 locus. Where needed, Sanger sequencing can be conducted. 2-4 weeks
Breeding Founders Breed the founders to wildtype rats and genotype their offspring to ensure transmission of the knockin allele. 12-16 weeks
Southern Blotting (Optional) Confirm knockin integration and gene copy numbers in founders or F1 progeny. 2-4 weeks
Note:
  • Standard services facilitate modifications up to 2kb. Projects requiring larger fragments may involve additional costs and extended timelines.
  • We primarily use SD and Long Evans rat strains, with options for customization.
Targeted Gene Editing Technology for Point Mutation Rats
Targeted Gene Editing Technology for Point Mutation Rats
Leverage our Targeted Gene Editing technology for precise point mutation in rats, ideal for modeling specific genetic disorders and studying gene function at the nucleotide level. This technique provides a high degree of accuracy in introducing targeted mutations that replicate human disease conditions.
Stage Description Turnaround Time
Strategy Design Develop a nuclease-mediated strategy tailored to your gene of interest, including gRNA and donor oligo design. 1-4 days
Vector Construction Construct vectors expressing the necessary nucleases and the oligo donor template; Where needed, efficacy tests in cell culture can be conducted. 3-4 weeks
Targeted Gene Editing Injection Co-inject ribonucleoprotein (RNP) and the donor oligo into fertilized mouse eggs, and implant to obtain founders. SD: 8-12 weeks, Long Evans: 10-16 weeks
Founder Screening Screen pups by PCR and Sanger sequencing to identify those with the correct point mutation knockin. 2-3 weeks
Off-Target Analysis Analyze potential off-target effects to ensure specificity of the gene edit. 1-2 weeks
Breeding Founders Breed the founders to wildtype rats and genotype their offspring by PCR and Sanger sequencing to ensure transmission of the knockin allele. 12-16 weeks
Note:
  • Turnaround times exclude institutional approval processes or shipping.
  • Our primary focus is on SD and Long Evans strains, but alternative strains can be accommodated on request.
FAQs
Frequently Asked Questions (FAQs)
What is a knockin rat and how is it different from a knockout rat?
A knockin rat has specific genes added or modified within its genome, typically to express new genes or introduce mutations for detailed genetic studies. This differs from a knockout rat, where specific genes are completely disabled or removed to study gene function loss and its effects on physiology and behavior.
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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