Each full PiggyBac-on-BAC transgenic mouse project is divided into the following phases:
1. PiggyBac-on-BAC transgenic strategy design
2. PiggyBac-on-BAC modification
3. PiggyBac-on-BAC transgenic pronuclear injection
4. PiggyBac-on-BAC transgenic mouse screening
Preparation of DNA for injection and development of a genotyping strategy
The success of PiggyBac-on-BAC transgenic production depends critically on the design and the quality of PiggyBac-BAC used in pronuclear injection. Accordingly, we employ a special protocol to design and prepare top-quality PiggyBac-BAC for our injections. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC.
Generation of PiggyBac-on-BAC transgenic founder mice
-PiggyBac-BAC injection to obtain founders, basic service: A minimum of 200 eggs (C57BL/6) will be injected to produce live animals. This will result in an average of 2 transgenic founders, but the actual numbers could fluctuate significantly, and it is possible that no founders are obtained. If you wish to ensure the generation of founders, we strongly recommend that you use our guaranteed service option (see below).
-PiggyBac-BAC injection to obtain founders, guaranteed service: We guarantee a minimum of 2 transgenic founders (the actual number of founders obtained is typically higher). We will inject as many eggs as needed to fulfill this guarantee.
-Genotyping pups to identify founders: Pups will be genotyped by PCR to identify PiggyBac-on-BAC transgenic founder mice following the strategy designed by Cyagen scientists.
We typically produce transgenic mice in C57BL/6 strain backgrounds, but we may be able to offer other strains to accommodate your special research needs.
|Service||Donor egg strain||Price||Turnaround time|
PiggyBac-on-BAC transgenic strategy design
|PiggyBac-BAC modification||Please inquire||2-5 weeks|
|Prepare PiggyBac BAC for injection and develop genotyping strategy||$450||1-2 weeks|
|PiggyBac-BAC injection to obtain founders, basic service||C57BL/6||$5,950||10-14 weeks|
|PiggyBac-BAC injection to obtain founders, guaranteed service||C57BL/6||$8,950||10-18 weeks|
|Genotyping pups to identify PiggyBac-on-BAC transgenic founders||$450||1 week|
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, mobidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.
If you find another commercial service provider that offers better pricing than ours, we will match the price plus an additional 5% off.
Standard payment terms include a 50% upfront payment before the project, and the remaining 50% plus shipping charge paid after completion of the project. If you need us to design your transgenic strategy, we will provide this service for free irrespective of whether you end up choosing us for your project.
We offer up to a 10% bulk discount for large orders. Large orders are defined as 5 or more projects from the same institution. If you bundle your orders with those of your colleagues, you can all qualify for the bulk discount.
Products are shipped from our Santa Clara, California facility. For mouse shipments, the shipping charge includes courier cost plus a $100/crate handling fee. DNA constructs or fixed/stained embryos are shipped at room temperature, and the charge includes courier cost plus a $10 handling fee. We typically use World Courier to ship live mice and FedEx for other shipments.
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