Each full transgenic project is split into the following phases:
1. Transgenic strategy design
2. Transgenic vector construction
3. Pronuclear injection
4. Transgenic mouse screening
Preparation of DNA for injection and development of a genotyping strategy
The success of transgenic production depends critically on the quality of DNA used in pronuclear injection. Accordingly, we employ a special protocol to prepare top-quality DNA for our injections. Plasmid DNA is typically linearized for injection. BAC DNA is typically injected in unlinearized form, as this has been shown to produce similar biological outcomes as linearized BAC. A PCR-based genotyping strategy will be developed and tested. If you provide your own transgenic vector rather than having us construct it, you can send us either E. coli or unlinearized DNA in solution (≥50 µg total), and we will perform the necessary preparation to purify/linearize the DNA. We strongly discourage customers from sending us linearized, injection-ready DNA, because the quality of such DNA is often insufficient for pronuclear injection. If you provide your own vector without sequence information, you will also need to provide a PCR-based genotyping strategy along with your vector.
Generation of transgenic founder mice
- Plasmid or BAC injection to obtain founders, basic service: A minimum of 400 eggs (C57BL/6) will be injected to produce live animals. This will result in an average of 2-3 transgenic founders in the case of plasmid injection or 1-2 founders in the case of BAC injection, but the actual numbers could fluctuate significantly and it is possible that no founders are obtained. If you wish to ensure the generation of founders, we strongly recommend that you use our guaranteed service option (see below).
- Plasmid or BAC injection to obtain founders, guaranteed service: We guarantee a minimum of 3 transgenic founders for plasmid injection or 2 founders for BAC injection (the actual number of founders obtained is typically higher).
- Genotyping pups to identify founders: Pups will be genotyped by PCR to identify founder mice.
We typically produce transgenic mice in C57BL/6 strain backgrounds, but we can use other strains per your request.
|Service||Donor egg strain||Price||Turnaround time|
|Transgenic strategy design||Free||1-4 days|
|Transgenic vector construction or BAC modification*||Please inquire||2-5 weeks|
|Prepare DNA for injection and develop genotyping strategy||$350||1-2 weeks|
|Plasmid injection to obtain founders, basic service||C57BL/6||$3,450||6-10 weeks|
|Plasmid injection to obtain founders, guaranteed service||C57BL/6||$3,950||6-16 weeks|
|BAC injection to obtain founders, basic service*||C57BL/6||$4,450||8-14 weeks|
|BAC injection to obtain founders, guaranteed service*||C57BL/6||$6,950||8-18 weeks|
|Genotyping pups to identify founders||$250||1 week|
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.
If you find another commercial service provider that offers better pricing than ours, we will match the price plus an additional 5% off.
Standard payment terms include a 50% upfront payment before the project, and the remaining 50% plus shipping charge paid after completion of the project. If you need us to design your transgenic strategy, we will provide this service for free irrespective of whether you end up choosing us for your project.
We offer up to a 10% bulk discount for large orders. Large orders are defined as 5 or more projects from the same institution. If you bundle your orders with those of your colleagues, you can all qualify for the bulk discount.
Products are shipped from our Santa Clara, California facility. For mouse shipments, the shipping charge includes courier cost plus a $100/crate handling fee. DNA constructs or fixed/stained embryos are shipped at room temperature, and the charge includes courier cost plus a $10 handling fee. We typically use World Courier to ship live mice and FedEx for other shipments.
All animal work is conducted in our specific pathogen free (SPF) facilities that have been AAALAC accredited and OLAW assured. For details information, please visit our support section for Description of our Facility, Animal Health and Animal Welfare Program.
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