Asian Pac J Cancer P 12:397 (2011)
RNA interference of caveolin-1 via lentiviral vector inhibits growth of hypopharyngeal squamous cell carcinoma FaDu cells In Vitro and In Vivo.
Xuening Zhao, Chao Ma, Xiaolan Cai, Dapeng Lei, Dayu Liu, Fenglei Xu, TongJin, Jun Liu, Xinliang Pan*
Objective: To investigate the effects of caveolin-1 (CAV1) on the growth of hypopharyngeal squamous cell carcinoma (HSCC) FaDu cells in vitro and in vivo.
Methods: A CAV1-RNAi-lentivirus construct was transfected into FaDu cells and expression of caveolin-1 was tested by RT-PCR and western blotting analysis. Cell apoptosis was analyzed by transferase-medisated dUTP nick-end labeling (TUNEL) assay. Tumor inhibition effects were investigated by injecting rCAV1-RNAi-lentivirus construct into tumors created with FaDu cells in the HSCC mouse model, with the empty-vector lentivirus as a control. CAV1 expression in xenografts was tested by RT-PCR and immunohistochemistry.
Results: RT-PCR and western blot analysis demonstrated successful construction of the CAV1-RNAi-lentivirus construct producing small hairpin RNA. The average weights and volumes of tumor in mice treated with CAV1-RNAi-lentivirus were lower than in mice with control treatment (P<0.05). RT-PCR revealed weak positive expression of CAV1 in CAV1-construct–treated xenografts and immunohistochemistry confirmed lower CAV1 expression than in controls.(P<0.05). In addition, downregulation of CAV1 increased cell apoptosis in vitro.
Conclusion: The growth of HSCCs could be inhibited by recombinant CAV1-RNAi-lentivirus in vitro and in vivo.