LD78-β/CCL3L1 is a CC chemokine that is closely related to MIP-1α. It signals through the CCR5 receptor and the β-chemokine receptor, D6. LD78-β has been shown to exhibit potent activity in HIV suppression assays.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Approximately 7.7 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Less than 1 EU/μg of LD78-β/CCL3L1 as determined by LAL method.
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/mL.
Sterile filtered white lyophilized (freeze-dried) powder.
APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA
> 97% by SDS-PAGE and HPLC analyses.