Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC
hyperplasia and atherosclerosis. The protein is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Murine HB-EGF shares about 81 % a.a. sequence identity with human HB-EGF.
10 μg / 50 μg / 1000 μg
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 500 mM NaCl, pH7.4.
Approximately 9.8 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
Less than 1 EU/μg of rMuHB-EGF as determined by LAL method.
> 97 % by SDS-PAGE and HPLC analyses.
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0× 10 6 IU/mg.
Sterile Filtered White lyophilized (freeze-dried) powder.
DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY
KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes.
FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
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