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人血小板衍生生长因子BB(PDGF-BB) (Human)

PDGF-BB(Human) 人血小板衍生生长因子BB(PDGF-BB) (Human)  HEGFP-1601
产品货号: HEGFP-1601
产品规格:
价格:


产品描述:
Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).


表达系统 :
Escherichia coli


溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.


产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.


存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.


分子量:
Approximately 12.3 kDa, a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain).


内毒素:
Less than 1 EU/μg of PDGF-BB as determined by LAL method.


产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.


生物学活性:
The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/mL, corresponding to a specific activity of > 3.3 × 105 IU/mg.


产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.


氨基酸序列:
MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT


产品纯度:
> 97% by SDS-PAGE and HPLC analyses.

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