Diabetes Metab Res Rev PMID: 24827928 (2014)

Effect of osteopontin in regulating bone marrow mesenchymal stem cells treatment on skin wounds in diabetic mice.

Meng H1, Wang Z, Wang W, Li W, Wu Q, Lei X, Ouyang X, Liang Z.



We aimed to investigate the role of osteopontin (OPN) in regulating MSCs transplanted to promote wound healing in diabetic mice.


The MSCs of OPN knock-out (KO) and wild-type (WT) mice were isolated separately for in vitro culture and characterization. A skinwound on the back of mice was established by skin punching. 27 OPN KO male mice were induced diabetic mellitus via intraperitoneal injection of streptozotocin (STZ) and 9 normal mice were as control. The mice were divided into 4 groups and injected DMEM medium or MSCs via the tail vein: A (diabetes injected with DMEM), B (diabetes injected with OPN KO MSCs), C (diabetes injected with WT MSCs), D(normal injected with DMEM). The healing times and closure rates of skin wounds were recorded. The microvessel density of healing wounds was measured, and the localized expression of OPN was identified by western blotting and immunohistochemistry. The migration of MSCs was observed on normal mice with skinwound injected MSCs of fluorescent mice.


Compared with normal mice, the healing time of wounds in the mice with diabetes and OPN KO was significantly prolonged (p < 0.01). After transplanting OPN KO MSCs the healing time was slightly shorter. Meanwhile, the healing time was significantly shorter after transplanted with WT MSCs, and more significant neovascularization at healing wounds (p < 0.05). The expression of OPN in local healing wounds after transplantation of WT MSCs were demonstrated with western blotting and immunohistochemistry. After 4 days, the green fluoresces were noted on the wounds ofmice injected MSCs of fluorescent mice.


MSCs can migrate to wound site and OPN plays a regulatory role in MSCs promoting the healing of diabetic skin wounds. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.

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