PLoS One 7:e29518 (2012)
Zeng Y, Wang W, Ma J, Wang X, Guo M, Li W.
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
The human gene encodes a typical KRAB-C2H2 zinc finger protein that may participate in hematopoiesis and leukemogenesis. A recent microarray study revealed that expression continuously decreases during erythropoiesis. However, the molecular mechanisms underlying regulation of during hematopoiesis are not well understood. Here we found that , a master regulator of erythropoiesis, repressed the promoter activity and transcription of . Electrophoretic mobility shift assays and chromatin immunoprecipitation assays showed that directly bound to a GATA binding site in the promoter in vitro and in vivo. of in erythroleukemia with specific siRNA accelerated cellular , suppressed apoptosis, and reduced expression of erythroid-specific developmental markers. It also promoted growth of subcutaneous -derived tumors in nude mice. These results suggest that is a crucial downstream target and effector of . They also suggest the downregulation of by is important in promoting the growth and suppressing the differentiation of erythroleukemia .