Chin J Cancer 29:952 (2010)

The Recruitment of Exogenous Endothelial Progenitor Cells in Lung Tumor Model of Nude Mice.

Qiang Peng1 , Ming Liu1 , MinSong Shu1 , HuaLi Xian2 , HuaDu Yi1 , Yong Zhi1 , MinYong Wang3


Backgroud and Objective:Endothelial progenitor cells(EPCs) play an important role in hypoxiatriggered tumor vasculogenesls. However, the homing of exogenous EPCs in tumor is still uclear. In this study, we investigated the recuitment of exogenous EPCs in human lung adenocarcinoma model of nude mice.

Methods:EPCs labeled with green fluorescence protein(GFP) were transplanted into nude mice bearing human lung adenocarcinoma. The growth of tumor was observed. After the mice were killed, GFP-EPCs in different tissues were examined by fluorescence.The tumor tissues were stained for CD133, hypoxia-inducible factor-1alpha(HIF-1a), stromal cell-derived factor-1a(SDF-1a), and vascular endorthelial growth factor receptor(KDR). Real-time polymerase chain reaction of CD133, HIF-1a,SDF-1a, and VEGF-1 were also performed.

Results: The growth of tumor in EPC group was significantly faster than that in saline solution group (P<0.05). Under fluorescence microscope, GFP-EPCs were strongly expressed in both tumor and bone marrow. EPCs were recruited to the tumor periphery to participate in tumor vasculogenesis. Theexpression of CD133, HIF1-a, and SDF-1 mRNA in tumor and bone marrow were significantly higher than that in the liver, spleen, and skin(P<0.05).

Conclusions: Exogenous EPCs can be recruited to rumor and acceleate tumor growth. Except tumor,bone marrow can also recruit EPCs.

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