J Biomater Appl 29:59 (2013)
Xia Y1, Peng SS2, Xie LZ1, Lian XJ3, Zhang XJ4, Cui H4, Song TX4, Zhang FM5, Gu N6, Cui FZ7.
To improve bone engineering for clinical applications, we coupled nanofiber-peptide hydrogel to nano-hydroxyapatite/collagen to form a bioactive scaffold (cnHAC) that mimics extracellular matrices. In comparison to nano-hydroxyapatite/collagen, we found that cnHAC promoted cell adhesion and spreading, and DNA content measurements, alkaline phosphatase activity assays, and reverse transcriptase-polymerase chain reaction analyses of osteogenic gene expression showed that cnHAC significantly improved cellular attachment, proliferation, and osteogenic differentiation in vitro (P < 0.05). In vivo models based on rat calvarial implants showed that cnHAC significantly enhanced bone regeneration (P < 0.05). In conclusion, we demonstrated that novel cnHAC scaffolds could potentially facilitate future bone regenerative medicine.