J Oral Pathol Med PMID: 24646032 (2014)
Liang QX1, Liang YC, Xu ZY, Chen WL, Xie HL, Zhang B.
Ameloblastoma is a frequent odontogenic neoplasm characterized by local invasiveness and high risk of recurrence. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a tumor suppressor that inhibits metastasis and angiogenesis. The aim of this study was to investigate effects of RECK overexpression on invasive potential in ameloblastoma cells.
Lentiviral vectors containing human RECK gene were created and subsequently stably transfected into immortalized ameloblastoma cell line hTERT+ -AM. Functional characteristics of hTERT+ -AM cells with stable RECK overexpression included proliferation, migration, invasion, and regulation of matrix metalloproteinases (MMP)-2, MMP-9 measured by zymography or commercially available assays.
The stable and higher expression of RECK mRNA and protein (P < 0.01) was detected in RECK-transfected hTERT+ -AM cells. RECKoverexpression caused a decrease in migration and invasion (P < 0.01) for hTERT+ -AM cells and a decrease in activity of MMP-2, MMP-9 (P < 0.01). Proliferation was not affected by RECK overexpression (P > 0.05).
Overexpression of RECK gene significantly inhibited cell invasive ability of hTERT+ -AM cells, suggesting RECK may be a new target for ameloblastoma treatment.
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
ameloblastoma; invasion; matrix metalloproteinase; reversion-inducing cysteine-rich protein with Kazal motifs