Targeted Gene Editing
Achieve precise genetic modifications in mice and rats with fast, cost-effective, and high-efficiency gene editing – powered by AI-optimized guide design and proprietary delivery techniques.
Wide Compatibility
Supports diverse model types across multiple strains and species.
High Editing Precision
AI-optimized guide molecules and enhanced HDR minimize off-target risks and improve editing fidelity.
Fast Turnaround
Accelerate your research with rapid model generation and efficient service delivery.
Technology
Applications
FAQs
How Targeted Gene Editing Works
Our Targeted Gene Editing platform introduces precise double-strand
breaks (DSBs) at specific genomic locations using AI-optimized guide
molecules. These breaks are repaired via homology-directed repair (HDR),
enabling the accurate insertion, deletion, or replacement of genetic
sequences.
Unlike traditional methods that primarily rely on non-homologous end
joining (NHEJ)—which often leads to small insertions or deletions
(indels)—our proprietary fertilization technique enhances HDR
efficiency, making it ideal for complex model generation such as
large-fragment knockin and conditional knockout.
Technology Workflow & Key Advantages
| Feature | Conventional Gene Editing | Cyagen’s Targeted Gene Editing |
|---|---|---|
| Editing Stage | Zygote or ES cell (varies) | Direct embryo-stage editing |
| Targeting Strategy | Random DSB repair (often NHEJ) | HDR-optimized with proprietary fertilization |
| Precision | May result in unpredictable indels | High-precision insertion/deletion/mutation |
| Model Complexity | Limited for large edits | Supports up to 15 kb knockin / 400 kb knockout |
| Guide Molecule Design | Manual or vendor tools | AI-powered (via AlphaKnockout) |
| Efficiency | Variable | Significantly enhanced via HDR boosting |
| Applicable Species | Limited strains | Mouse (C57BL/6, FVB), Rat (SD, Wistar, others) |
Looking for higher success rates and complex model capabilities?
Explore our TurboKnockout® platform →
What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
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