Logo
Homepage
Explore Our Models
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Cell Immunotherapy CRO Services
From antibody discovery to in vivo validation, Cyagen delivers an end-to-end solution for immune cell therapy R&D — supporting CAR-T, CAR-NK, and universal cell therapies with customizable services spanning antibody development, CAR vector design, cell engineering, and efficacy testing to empower breakthroughs in oncology and beyond.
One-Stop Autoimmune & Inflammation CRO Solutions
Cyagen provides one-stop CRO solutions for autoimmune and inflammation drug discovery, offering both genetically engineered and induced disease models alongside full-spectrum in vivo and in vitro efficacy services. With extensive experience in modeling RA, SLE, IBD, and other disorders, our platform supports both basic research and translational studies — delivering data-driven insights for IND submissions and therapeutic innovation.
{{ item.title }}
{{static_tabs[0].title}}
Antibody Discovery for CAR Constructs
Dual Strategies for scFv Generation – Built for Flexibility
Whether your research calls for a robust in vivo immune response or high-throughput in vitro selection, Cyagen provides two complementary approaches to antibody generation for CAR molecule design. Choose from HUGO-Ab™ fully human antibody mice for natural repertoire development, or our phage display libraries for rapid screening of high-affinity binders — both backed by expert validation and seamless CAR construct integration.
{{ item.label }}
{{v2.label}}
{{item.context}}
{{v2.context}}
{{item.title}}
{{ item.context }}
{{v2.title}}
  • {{v3}}
{{item?.link?.text}}
{{static_tabs[1].title}}
CAR Molecule Design & Vector Engineering
Tailor Your CAR Construct with Confidence
Cyagen provides comprehensive support for chimeric antigen receptor (CAR) molecule design and lentiviral vector construction. From scFv integration to co-stimulatory domain selection and reporter tagging, our team helps you develop functional, customizable
Key Capabilities:
  • Modular CAR Design: scFv screening and optimization, hinge/transmembrane/co-stimulatory domain selection (e.g., CD28, 4-1BB)
  • Vector Construction: Gene synthesis and subcloning into 3rd-generation lentiviral backbones
  • CAR Reporter Integration: Optional GFP, RFP, or luciferase tagging for expression validation
  • Ready-to-Use Plasmid Libraries: Available constructs targeting CD19, GPC3, BCMA, HER2, etc.
Figure 1. Modular Structure of a CAR Molecule
Figure 2. Lentiviral Vector Map for CAR Expression
Deliverables:
  • Vector maps and sequence files
  • CAR expression plasmids
  • Lentiviral packaging-ready constructs
  • QC report on vector integrity and CAR cassette structure
{{static_tabs[2].title}}
Immune Cell Preparation
Engineered for Precision and Performance
Cyagen provides high-quality preparation of CAR-T, CAR-NK, and universal CAR-T (UCAR-T) cells using standardized and customizable protocols. From primary cell isolation to CAR transduction and expansion, every step is optimized to ensure high expression, p
Key Capabilities:
  • T and CAR-T Cell Production: CD4/CD8-balanced populations with high viability and transduction efficiency
  • NK and CAR-NK Cell Generation: High-activity innate effector cells suitable for allogeneic therapy
  • Universal CAR-T Cell Engineering: TCRα/CD52 knockout with CRISPR and lentiviral transduction for GVHD-free UCAR-T cells
  • Murine CAR-T Cells: For use in murine tumor models or immunological research
  • Immortalized CAR Tool Cells: Jurkat, NK-92, and THP-1 engineered for CAR screening and assay standardization
Deliverables:
  • Validated CAR+ immune cells (T, NK, UCAR-T, etc.)
  • Cell purity and positivity reports
  • Full experimental documentation
{{static_tabs[3].title}}
In Vitro Efficacy Evaluation
Functional Insights at Every Step
Cyagen’s in vitro assays provide robust pharmacological data to support the evaluation of CAR-T, CAR-NK, UCAR-T, and related cell therapies. From cytotoxicity to cytokine secretion, our in vitro services are designed to validate candidate potency, optimize dosing strategies, and guide in vivo studies with confidence.
Key Capabilities:
  • Cell Proliferation Assays: Quantify CAR cell expansion under various stimulatory conditions
  • Cytotoxicity Assays: Co-culture with tumor cell lines to assess real-time killing activity
  • Cytokine Release Profiling: Analyze secreted IFN-γ, TNF-α, IL-2 and others post-target engagement
  • Phagocytosis Assays: Assess CAR-macrophage antibody-dependent cellular phagocytosis (ADCP)
  • ADCC/ADCP Assays for Antibody Drugs: Evaluate immune effector function of mAbs with NK/macrophage models
Deliverables:
  • Cell proliferation curves and viability metrics
  • Cytotoxicity data with target cell killing efficiency
  • Cytokine concentration results (ELISA or multiplex)
  • Imaging or flow-based phagocytosis analysis
  • Comprehensive experimental report
{{static_tabs[4].title}}
In Vivo Pharmacology
Translate Cell Function into Tumor Response
Cyagen offers comprehensive in vivo pharmacology solutions for evaluating immune cell therapy efficacy in preclinical models. Our services span model selection, drug administration, and multi-parameter efficacy assessment — enabling robust and reproducible
CDX & Syngeneic Tumor Models
Leverage our tumor cell line database and modeling expertise to assess CAR-T/NK therapies in immunodeficient (e.g., NKG) or immunocompetent hosts.
→ Common models: subcutaneous, intravenous, orthotopic implants
HIS (Human Immune System) Models
Use PBMC or HSC humanized mice to simulate human immune responses for universal and allogeneic CAR cell products.
→ Long-window, high-homogeneity models with proven engraftment performance
Drug Administration & Tumor Monitoring
Standardized protocols for:
  • Immune cell infusion
  • Tumor volume tracking (caliper or bioluminescence)
  • Survival and body weight monitoring
Biochemical & Histological Analysis
Evaluate treatment efficacy through:
  • Blood cytokine profiling (e.g., IFN-γ, IL-2)
  • Tumor tissue histology and IHC
  • Immune cell infiltration markers
Deliverables:
  • Tumor growth curves, survival and body weight data
  • Imaging data from bioluminescent/luciferase-labeled tumors
  • Cytokine levels and immune cell infiltration analysis
  • Full study report with statistical analysis and interpretation
Case Highlights
{{ item.label }}
{{v2.label}}
{{item.context}}
{{item?.iamges}}
{{v2.context}}
{{item.title}}
{{ item.context }}
{{v2.title}}
  • {{v3}}
{{item?.link?.text}}
Our Unique Advantages
End-to-End Customization Across Development Pipeline
From antibody discovery to in vivo validation, Cyagen offers modular services supporting CAR-T, CAR-NK, and universal therapies — accelerating development with seamless integration.
Proprietary Models & Toolkits for In Vivo Evaluation
Leverage our proprietary NKG mice, validated CDX/syngeneic tumor models, and HIS (PBMC/HSC) platforms for reproducible, translational in vivo data.
Robust Cell Engineering with GMP-Ready Workflows
We deliver high-purity, high-activity CAR-T/NK cells using optimized protocols — including TCRα/CD52 knockout and scalable lentiviral transduction — built for reliability and consistency.
Request a Preclinical CRO Services Consultation
Partner with Cyagen to advance your preclinical studies. Share your project goals with us and receive customized support.
Inquiry Details
Main Area of Research
Service(s) of Interest
Project Details
Contact Information
Full Name
Email
Phone Number
Organization
Job Role
Country
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our  Privacy Policy  for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest