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Standard AAV Packaging
Cyagen offers high-purity, high-titer AAV vectors for gene therapy and in vivo research. Our optimized three-plasmid system ensures high infection efficiency, low endotoxin levels, and broad serotype compatibility.
High Purity & Titer
Achieves robust gene expression in dividing and non-dividing cells.
Broad Serotype Selection
Offers multiple serotypes for different tissues and applications.
Fast & Reliable Delivery
Standard AAV projects ship in as fast as 2 weeks with rigorous QC testing.
Overview
Workflow
FAQs
Overview
Custom AAV Packing Services
To ensure the reliability of viral packaging, we maintain strict control over viral titer, purity, and functional activity. Our AAV production employs a three-plasmid co-transfection system with multiple serotype options. Each batch achieves >95% purity with endotoxin levels <10 EU/mL. We further guarantee viral quality and performance through in-house validation, where our technical team evaluates infectivity, specificity, and transduction efficiency in both cultured cells and mouse models. This comprehensive workflow provides strong assurance of AAV quality and experimental consistency.
Service Type Group Titer Turnaround Time
Plasmid Construction Gene synthesis required \ 2-4 weeks
No gene synthesis required \ 1-3weeks
AAV Packing 1×1012 vg ≥5×1012 vg/ml As fast as 2 weeks
5×1012 vg ≥1×1013 vg/ml
1×1013 vg ≥1×1013 vg/ml
2×1013 vg ≥1×1013 vg/ml
5×1013 vg ≥1×1013 vg/ml
1×1014 vg ≥1×1013 vg/ml
others others
Browse In-Stock AAV →
Data Validation Highlights:
Figure 1. Schematic Diagram of AAV Vector Quality Control Results
Workflow
Workflow and Delivery
Cyagen’s AAV packaging service ensures high-purity, high-titer virus production with customizable options tailored for gene therapy and biomedical research. Below is an overview of our streamlined workflow, service specifications, and available serotypes.
AAV Technical Workflow
Service Comparison & Deliverables
We provide multiple grades of AAV packaging services, customized for diverse applications to guarantee optimal performance and strict biosafety compliance.
AAV Type Production Scale Virus Amount Titer Turnaround QC & Deliverables
Overexpression Pilot ≥1×10¹² vg ≥5×10¹² vg/mL 5-8 weeks - Purified AAV particles (selected serotype)
- Complimentary control virus
- qPCR-based titer quantification
- SDS-PAGE & Western blot validation
- Endotoxin testing (<10 EU/mL)
- Purity validation (≥95%)
Medium ≥5×10¹² vg ≥1×10¹³ vg/mL
Large ≥1×10¹³ vg ≥1×10¹³ vg/mL
RNA Interference Pilot (3+1) ≥1×10¹² vg ≥5×10¹² vg/mL 3-5 weeks
Medium (3+1) ≥5×10¹² vg ≥1×10¹³ vg/mL
Large ≥1×10¹³ vg ≥1×10¹³ vg/mL
Knockout Pilot ≥1×10¹² vg ≥5×10¹² vg/mL 3-5 weeks
Medium ≥5×10¹² vg ≥1×10¹³ vg/mL
Large ≥1×10¹³ vg ≥1×10¹³ vg/mL
FAQs
Frequently Asked Questions (FAQs)
What is the typical titer range of your standard AAV packaging?
We guarantee standard AAV titers: ≥5 × 10¹² vg/mL for small-scale packages and ≥1 × 10¹³ vg/mL for other package sizes. Custom titers are available upon
Which AAV serotypes do you offer?
We provide AAV1, AAV2, AAV5, AAV6, AAV7, AAV8, AAV9, and more upon request.
How do you ensure quality control for AAV packaging?
By default, we provide a target gene plasmid sequencing report and a viral titer certificate. For projects requiring enhanced characterization, we offer a comprehensive suite of additional Quality Control (QC) assays, including: endotoxin detection (LAL assay), mycoplasma testing (PCR method), sterility testing (direct culture method), capsid protein analysis via SDS-PAGE (Silver/Coomassie Blue staining), TEM analysis (providing an empty capsid ratio report), and viral genomic DNA purity assessment (agarose gel electrophoresis).
Can you produce custom AAV vectors for specific applications?
Yes, we offer AI-enhanced custom AAV variant design and packaging.
How long does it take to receive my AAV vectors?
Standard orders can be delivered within as fast as 2 weeks. Custom projects may require 3–8 weeks.
Citation Database
Molecular Therapy: Methods & Clinical Development, March, 2025
Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing
【Other】
Gut, February, 2025
E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression
【Other】
Cell Death & Disease, February, 2025
Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage
【Other】
Molecular Therapy, February, 2025
Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer
【Other】
Contact Us
Connect with our experts for your viral vector packaging needs. Please fill out the form below to start a conversation or request a quote.
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