Custom Rodent Gene Editing Models

From gene disruption to precise knockin, humanization, reporter insertion, and transgenic expression, Cyagen delivers custom genetically engineered rodent models designed for reliable disease modeling, gene function studies, and preclinical research.

One Integrated Platform for Rodent Genome Engineering

Translate Your Research Question

Rodent gene editing is not a one-size-fits-all process. Different research goals require different engineering strategies, from deleting a critical exon to inserting a human disease mutation, replacing a rodent gene with its human counterpart, or introducing a transgene for controlled expression studies.

Instead of separating projects by technology name, Cyagen focuses on the biological outcome you need. Our team helps translate your research question into the right model design.

Research Objective	Recommended Animal Model	Schematic diagram
Investigating fundamental gene functions, exploring disease mechanisms, or validating new therapeutic targets.	Conventional Knockout Model
Circumventing embryonic lethality, exploring tissue-specific biology, or triggering inducible disease states.	Conditional Knockout Model
Replicating patient-specific genetic alterations, rare variants, or targeted disease-associated point mutations.	Point Mutation/Precise Knockin Model
Assessing translational pharmacology, testing biologics, or studying targets unique to human biology.	Humanized Model
Monitoring cellular lineages, mapping pathway activation, or observing gene expression dynamics.	Reporter Knockin Model
Replicating intricate multi-domain gene structures, extensive human genetic loci, or broad regulatory sequences.	Large-Fragment Knockin or BAC-Based Model
Investigating the effects of gene overexpression, assessing enhancer functions, or modeling transgene-driven phenotypes.	Transgenic Model
Ensuring robust and stable transgene expression during preclinical model development.	PiggyBac Transgenic Model

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

Why Choose Cyagen for Rodent Gene Editing?

Our team helps match your research objective to the right model type, whether the best approach is knockout, conditional knockout, knockin, humanization, reporter insertion, or transgenic expression.

Cyagen combines targeted gene editing, ES cell-based strategies, TurboKnockout™ technology, and transgenic model generation to support diverse project requirements.

From point mutations and safe harbor knockins to conditional alleles and large-fragment insertions, we support complex rodent genome engineering projects.

Standard services are commonly performed in C57BL/6, with additional strain options available depending on the project design.

Projects can include genotyping, sequencing, founder screening, breeding, germline transmission confirmation, and delivery of study-ready animals.

Cyagen can support the full process from strategy design to final cohort delivery, helping reduce technical burden and accelerate research timelines.

Workflow and Delivery

Cyagen provides end-to-end rodent gene editing support, with flexible entry points depending on your project stage. We can manage the full workflow or support selected phases such as vector construction, embryo injection, founder screening, breeding, validation, and cohort delivery.

Notes

Turnaround times: Estimated turnaround times exclude periods required for host institution approval, rodent importation approval, shipping, or transit.
Pricing and custom requests: Pricing details are available upon inquiry. For specific strain requests, larger knockin fragments, extended breeding, or other custom requirements, please contact us for detailed information and pricing.
Strain information: Standard services are primarily performed in the C57BL/6 or SD strain. Other strains may be available upon request.
Knockin fragment size: Standard knockin services support fragment insertion of up to 8 kb. Larger fragments may require additional time, cost, and customized project evaluation.
Embryo-based transgenic options: Transgenic rodent embryos, including PiggyBac transgenic rodent embryos where applicable, are available for projects focused on early developmental or embryonic gene function studies, such as enhancer analysis. This provides a streamlined option for studies that do not require maintaining a full rodent line.

->Start Your Project

Frequently Asked Questions (FAQs)

A rodent gene editing model is a genetically engineered rodent in which a specific gene or genomic sequence has been deleted, inserted, replaced, modified, or externally introduced. These models help researchers study gene function, disease mechanisms, therapeutic targets, and drug response in vivo.

Knockout models disrupt or remove gene function. Knockin models introduce a defined sequence change or insertion at a specific genomic locus. Transgenic models introduce exogenous genetic material to express a new trait or study gene regulation. In practice, these are different model outcomes within the broader field of rodent gene editing.

A conditional knockout model is recommended when complete gene deletion may cause embryonic lethality, systemic toxicity, or unclear phenotypes. Conditional strategies allow gene inactivation in selected tissues, cell types, or developmental stages.

Safe harbor loci such as ROSA26 or H11 are often used when researchers need stable transgene expression while minimizing disruption to endogenous gene function. This approach is suitable for reporter expression, human gene expression, and controlled model development.

Yes. Cyagen supports complex gene editing strategies, including large-fragment knockin and BAC-based transgenic approaches. The recommended method depends on the insert size, target locus, expression requirements, and downstream study goals.

C57BL/6 and SD are commonly used as a standard background, while other strain options may be available depending on the specific model design, technical route, and project requirements.

Useful starting information includes the target gene, desired modification, reference sequence, preferred strain, intended research application, expected deliverables, and whether you need founders, F1 animals, validated lines, or study-ready cohorts.

Yes. Cyagen can review your research objective and recommend the most suitable model strategy, including knockout, conditional knockout, knockin, humanized, reporter, regular transgenic, or PiggyBac transgenic approaches.

Publication

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Knockout Mice

Mice with targeted gene deletions for gene function and disease studies.

Knockin Mice

Mice with specific genes inserted to study gene functions and disease.

Transgenic Mice

Mice with extra genes to study gene expression and its role in diseases.

Knockout Rats

Rats with gene knockouts to investigate gene function and disease models.

Knockin Rats

Rats with inserted genes for advanced functional studies and disease research.

Transgenic Rats

Rats with additional genes to explore gene expression and disease mechanisms.

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