BAC Modification Services
Cyagen provides comprehensive BAC modification services—from reporter insertion and point mutations to plasmid subcloning and selection marker integration. Whether you’re developing transgenic models or dissecting regulatory regions, we guarantee accurate, validated results with rapid turnaround.
Full Workflow Covered
From sourcing to validation—we handle it all.
Scarless or FRT Options
Flexible one or two-step workflows to suit your study.
Guaranteed Custom Edits
Expert-designed BAC modifications with delivery assurance.
Overview
Workflow
FAQs
Tailored BAC Editing with Reliable Validation
Cyagen offers robust BAC recombineering solutions for adding reporters, introducing point mutations, or simplifying BAC-based constructs. We ensure high-fidelity modifications with rigorous sequencing validation and can even generate transgenic mice from your modified BAC—making us a true one-stop partner for large-fragment genomic engineering.
Service Types and Descriptions:
1. BAC Design & Strategy
Custom modification plan based on your research goals.
2. Reporter or Mutation Insertion
Insert GOI (e.g., lacZ, GFP) or introduce point mutations via recombineering.
3. BAC-to-Plasmid Subcloning
Transfer up to 60 kb of BAC sequence into plasmid backbone.
4. Marker Integration
Add drug-selection or visualization marker to BAC backbone.
5. Scarless Two-Step Editing
High-precision modifications with no residual sequences.
6. Sequence Validation & QC
Full sequencing and PCR-based integrity checks.
Service Types and Descriptions:
1. BAC Design & Strategy
Custom modification plan based on your research goals.
2. Reporter or Mutation Insertion
Insert GOI (e.g., lacZ, GFP) or introduce point mutations via recombineering.
3. BAC-to-Plasmid Subcloning
Transfer up to 60 kb of BAC sequence into plasmid backbone.
4. Marker Integration
Add drug-selection or visualization marker to BAC backbone.
5. Scarless Two-Step Editing
High-precision modifications with no residual sequences.
6. Sequence Validation & QC
Full sequencing and PCR-based integrity checks.
Explore Ready-to-Use Mouse Models
Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.
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What Customers Say About Cyagen
Violet Shimmer
Stanford University
The service provided to us by Cyagen is now in press at Nature as an article.
Scarlett Rouge
Seattle Children’s Hospital
We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.
Contact Us
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