What are transgenic rats and how are they used in research?

Transgenic rats are genetically engineered to include foreign DNA, allowing researchers to study gene function, regulation, and expression. These models are crucial for understanding human diseases, testing pharmaceuticals, and exploring treatments in conditions that mimic human biology.

Custom Transgenic Rat Models for Reliable Gene Research

Transgenic Rats

Discover Cyagen's advanced transgenic rat services, utilizing cutting-edge methods for precise genetic modifications. Our established Regular and PiggyBac transgenesis techniques offer robust and dependable gene integration solutions, perfect for complex disease modeling and functional studies.

100% Genotype Match Guarantee

Get animals with correct genotype or your money back.

Streamlined Efficiency

Cost-effective gene targeting through mature, streamlined processes.

Diverse Strain Availability

Access strains such as SD, Wistar, LE, F344, Norway, Lewis, and more.

Overview

Workflow

FAQs

Overview

Comprehensive Transgenic Rat Models

Cyagen provides pioneering transgenic rat services, employing both Regular and PiggyBac technologies for diverse genetic integration needs. Our services range from using plasmid or BAC vectors in Regular Transgenic rats for extensive gene function analysis, to employing PiggyBac technology for uniform expression through single-copy gene insertion, ideal for intricate disease research.

Leverage our expertise for swift, cost-effective, and reliable transgenic rat model generation, specifically designed to meet the comprehensive demands of your scientific inquiries.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

What Services Are Offered for Transgenic Rats

Regular Transgenesis

Pronuclear injection method for high-expression, multi-copy transgenes

PiggyBac Transgenesis

Single-copy insertion system for stable, reproducible gene expression

Rodent Phenotyping

Full-spectrum analysis for rodents model

Rodent Breeding

Scalable colony expansion with full genotyping support

BAC Modification

Custom genomic edits with full validation and transgenic support

Cryopreservation & Recovery

Preserve and revive rodent strains on demand

Workflow

Workflow and Delivery

Our robust workflow ensures the efficient creation and delivery of both Regular and PiggyBac transgenic rats. By integrating advanced genetic technologies, we facilitate precise transgene integration tailored to your specific research needs. From initial design through to delivery, our processes are structured to accelerate your projects with high-quality, dependable transgenic models.

Regular Transgenic Rats: Plasmid-Based

Delve into our streamlined Regular Transgenic services using plasmid vectors. This approach is tailored to efficiently incorporate foreign DNA into the rat genome for varied gene function studies and disease modeling.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Develop a customized transgenic strategy including a PCR genotyping plan for founder screening.	1-4 days
Vector Construction	Construct and confirm plasmid-based transgenic vectors by restriction digest and sequencing.	2-5 weeks
Pronuclear Injection	Inject transgenic plasmid into the pronucleus of fertilized eggs and implant into surrogate mothers.	SD: 7-11 weeks, Long Evans: 11-15 weeks
Transgenic Founders Screening	Genotype pups by PCR to identify those carrying the transgene.	1 week

Note:

Turnaround times do not include periods required for institutional approval or transit. For specific strain requests or more detailed information, please inquire. We also offer transgenic rat embryos for projects focused on early developmental gene function studies, such as enhancer analysis, eliminating the need to maintain a full rat line.

Regular Transgenic Rats: BAC-Based

Explore our advanced BAC-based Regular Transgenic services, designed for the integration of large DNA constructs. This method utilizes BAC (Bacterial Artificial Chromosome) vectors for higher fidelity in genetic modifications, suitable for complex gene function analysis and detailed disease modeling.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Design a comprehensive transgenic strategy tailored to your research needs.	1-4 days
BAC Modification (Recombineering)	Modify BAC vectors as needed for the transgene; inquire for details on BAC recombineering services.	3-6 weeks
Prepare DNA for Injection	Purify and prepare BAC DNA for injection, develop a PCR-based genotyping strategy.	1-2 weeks
BAC Injection to Obtain Founders	Inject BAC into fertilized eggs and implant them to obtain transgenic founders; basic and guaranteed service options available.	SD: 10-16 weeks, Long Evans: 14-20 weeks
Transgenic Founders Screening	Screen pups by PCR to identify those carrying the BAC transgene.	2-3 weeks

Note:

Turnaround times exclude the period required for obtaining host institution’s approval and transit. For specific strain requests or further details, please inquire. We also offer transgenic rat embryos for use in early developmental gene function studies, providing a streamlined option for projects that do not require the maintenance of a full rat line.

PiggyBAC Transgenic Rats: Plasmid-Based

Explore the PiggyBAC transgenic method using plasmid vectors for precise and stable gene integration.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Design a comprehensive transgenic strategy tailored to your research needs.	1-4 days
BAC Modification (Recombineering)	Modify BAC vectors as needed for the transgene; inquire for details on BAC recombineering services.	3-6 weeks
Prepare DNA for Injection	Purify and prepare BAC DNA for injection, develop a PCR-based genotyping strategy.	1-2 weeks
BAC Injection to Obtain Founders	Inject BAC into fertilized eggs and implant them to obtain transgenic founders; basic and guaranteed service options available.	SD: 10-16 weeks, Long Evans: 14-20 weeks
Transgenic Founders Screening	Screen pups by PCR to identify those carrying the BAC transgene.	2-3 weeks

Note:

Turnaround times exclude periods required for obtaining host institution’s approval and transit. For specific strain requests or detailed information, please inquire. We also provide PiggyBAC transgenic rat embryos for research requiring only embryonic gene function studies, simplifying the process and reducing the need for full rat line maintenance.

PiggyBAC Transgenic Mice: BAC-Based

Experience the precision of PiggyBAC transgenic technology for BAC-based integrations. Our workflow ensures high fidelity and stability in gene expression, optimizing your research outcomes.

Stage	Description	Turnaround Time
BAC Transgenic Strategy Design	Customize a PiggyBAC-on-BAC transgenic strategy including genotyping protocols.	1-4 days
BAC Modification	Modify BACs through recombineering, validate modifications by restriction digest and sequencing.	3-6 weeks
BAC Pronuclear Injection	Inject modified BAC into the pronucleus of fertilized eggs and implant into surrogate mothers.	SD: 10-16 weeks, Long Evans: 14-20 weeks
Transgenic Founders Screening	Screen pups by PCR to identify those carrying the PiggyBAC-on-BAC transgene.	2 weeks

Note:

Turnaround times do not include periods required for institutional approval or transit. For specific strain requests or more information, please inquire. We also offer PiggyBAC transgenic rat embryos for researchers focusing on embryonic gene function studies without the need for maintaining a full rat line.

FAQs

Citation Database

Molecular Therapy: Methods & Clinical Development, March, 2025

Intracranial AAV administration dose-dependently recruits B cells to inhibit the AAV redosing

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E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression

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Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage

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