Transgenic Mice

Discover Cyagen's transgenic mouse services, offering both Regular and PiggyBac methods for diverse research applications. Benefit from our mature technology for gene integrations and tailored modifications that enhance disease modeling and functional studies, ensuring reliable results.

100% Genotype Match Guarantee

Get animals with correct genotype or your money back.

Streamlined Efficiency

Cost-effective gene targeting through mature, streamlined processes.

Swift Turnaround Time

Get your gene targeting mouse models faster.

Overview

Workflow

FAQs

Overview

Comprehensive Transgenic Mouse Models

Cyagen offers advanced transgenic mouse services, utilizing both Regular and PiggyBac technologies for gene integration. Our models cater to a variety of research needs, from Regular Transgenic mice using plasmid or BAC vectors for broad gene function analysis, to PiggyBac models designed for uniform expression through single-copy gene insertion, ideal for detailed disease research.

Leverage our expertise for rapid, cost-effective, and reliable model generation, specifically tailored to meet the broad demands of your research projects.

Explore Ready-to-Use Mouse Models

Discover over 18,000 validated mouse strains—including knockout, conditional knockout, and humanized models—covering 20+ research areas such as oncology, neurology, and metabolism. All models are supported by detailed genotype data and guaranteed quality, helping you fast-track discovery with confidence.

What Services Are Offered for Transgenic Mice

MouseAtlas Model Library

Search and access curated genetically engineered mouse strains

Regular Transgenesis

Pronuclear injection method for high-expression, multi-copy transgenes

PiggyBac Transgenesis

Single-copy insertion system for stable, reproducible gene expression

Rodent Breeding

Scalable colony expansion with full genotyping support

BAC Modification

Custom genomic edits with full validation and transgenic support

Rodent Phenotyping

Full-spectrum analysis for rodents model

Workflow

Workflow and Delivery

Our robust workflow ensures the efficient creation and delivery of both Regular and PiggyBAC transgenic mice. Utilizing advanced genetic technologies, we facilitate effective transgene integration tailored to your specific research demands. From conception through delivery, our processes are designed to accelerate your research with high-quality, reliable transgenic models.

Regular Transgenic Mice: Plasmid-Based

Delve into our streamlined Regular Transgenic services using plasmid vectors. This approach is tailored to efficiently incorporate foreign DNA into the mouse genome for varied gene function studies and disease modeling.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Develop a customized transgenic strategy including a PCR genotyping plan for founder screening.	1-4 days
Vector Construction	Construct and confirm plasmid-based transgenic vectors by restriction digest and sequencing.	4-6 weeks
Pronuclear Injection	Inject transgenic plasmid into the pronucleus of fertilized eggs and implant into surrogate mothers.	8-12 weeks
Transgenic Founders Screening	Genotype pups by PCR to identify those carrying the transgene.	8-12 weeks

Note:

Turnaround times do not include periods required for institutional approval or transit. For specific strain requests or more detailed information, please inquire. We also offer transgenic mouse embryos for projects focused on early developmental gene function studies, such as enhancer analysis, eliminating the need to maintain a full mouse line.

Regular Transgenic Mice: BAC-Based

Explore our advanced BAC-based Regular Transgenic services, designed for the integration of large DNA constructs. This method utilizes BAC (Bacterial Artificial Chromosome) vectors for higher fidelity in genetic modifications, suitable for complex gene function analysis and detailed disease modeling.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Design a comprehensive transgenic strategy tailored to your research needs.	1-4 days
BAC Modification (Recombineering)	Modify BAC vectors as needed for the transgene; inquire for details on BAC recombineering services.	8-10 weeks
Prepare DNA for Injection	Purify and prepare BAC DNA for injection, develop a PCR-based genotyping strategy.	1-2 weeks
BAC Injection to Obtain Founders	Inject BAC into fertilized eggs and implant them to obtain transgenic founders; basic and guaranteed service options available.	8-12 weeks
Transgenic Founders Screening	Screen pups by PCR to identify those carrying the BAC transgene.	8-12 weeks

Note:

Turnaround times exclude the period required for obtaining host institution’s approval and transit. For specific strain requests or further details, please inquire. We also offer transgenic mouse embryos for use in early developmental gene function studies, providing a streamlined option for projects that do not require the maintenance of a full mouse line.

PiggyBAC Transgenic Mice: Plasmid-Based

Explore the PiggyBAC transgenic method using plasmid vectors for precise and stable gene integration. This technique is renowned for its efficiency in generating transgenic mice with consistent gene expression.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Develop a PiggyBAC transgenic strategy tailored to your research objectives, including a PCR genotyping plan for founder screening.	1-4 days
Vector Construction	Construct and verify PiggyBAC transgenic plasmids, preparing them for injection.	8-10 weeks
Pronuclear Injection	Inject PiggyBAC plasmids into the pronucleus of fertilized eggs and implant them into surrogate mothers to obtain offspring.	8-12 weeks
Transgenic Founders Screening	Genotype pups by PCR to identify those carrying the PiggyBAC transgene.	8-12 weeks

Note:

Turnaround times exclude periods required for obtaining host institution’s approval and transit. For specific strain requests or detailed information, please inquire. We also provide PiggyBAC transgenic mouse embryos for research requiring only embryonic gene function studies, simplifying the process and reducing the need for full mouse line maintenance.

PiggyBAC Transgenic Mice: BAC-Based

Experience the precision of PiggyBAC transgenic technology for BAC-based integrations. Our workflow ensures high fidelity and stability in gene expression, optimizing your research outcomes.

Stage	Description	Turnaround Time
Transgenic Strategy Design	Develop a PiggyBAC transgenic strategy tailored to your research objectives, including a PCR genotyping plan for founder screening.	1-4 days
Vector Construction	Construct and verify PiggyBAC transgenic plasmids, preparing them for injection.	8-10 weeks
Pronuclear Injection	Inject PiggyBAC plasmids into the pronucleus of fertilized eggs and implant them into surrogate mothers to obtain offspring.	8-12 weeks
Transgenic Founders Screening	Genotype pups by PCR to identify those carrying the PiggyBAC transgene.	8-12 weeks

Note:

Turnaround times do not include periods required for institutional approval or transit. For specific strain requests or more information, please inquire. We also offer PiggyBAC transgenic mouse embryos for researchers focusing on embryonic gene function studies without the need for maintaining a full mouse line.

FAQs

What Customers Say About Cyagen

Violet Shimmer

Stanford University

The service provided to us by Cyagen is now in press at Nature as an article.

Scarlett Rouge

Seattle Children’s Hospital

We are very pleased with the state-of-the-art professional transgenic services provided by Cyagen for our study published recently in Nature. We continue to use Cyagen’s transgenic services as it allows us to do better and more efficient research with transgenic mice.

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