Alb-cre+/MYC+ Mouse
Product Name
Alb-cre+/MYC+ Mouse
Product ID
C001339
Strain Name
C57BL/6JCya-lgs2em1(CAG-LSL-HA tag-hMYC-IRES-eGFP)Tg(Alb-Cre)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “Alb-cre+/MYC+ Mouse (Catalog C001339) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
MYC
Gene Alias
MRTL, MYCC, c-Myc, bHLHe39
NCBI ID
Chromosome
Chr 8 (Human)
MGI ID
Datasheet
Strain Description
The MYC oncogene family comprises regulatory genes and proto-oncogenes that encode transcription factors, involved in various cellular processes such as the cell cycle, apoptosis, DNA repair, and metabolism. Members include c-Myc (MYC), l-Myc (MYCL), and n-Myc (MYCN). c-Myc (MYC) is a basic helix-loop-helix leucine zipper (bHLHZip) transcription factor, which forms heterodimers with Max protein to bind DNA and regulate the expression of approximately 15% of genes, thereby participating in key cellular processes such as cell proliferation, apoptosis, DNA repair, and metabolism. In many cancers, c-Myc is overexpressed, leading to uncontrolled cell proliferation and tumor growth, such as in Burkitt's lymphoma where c-Myc gene rearrangement is common. Dysregulation of the MYC oncogene plays a crucial role in tumorigenesis, predominantly through transcriptional dysregulation resulting in overexpression of c-Myc protein.
Alb-Cre+/MYC+ mice are generated by crossing H11-CAG-LSL-hMYC-IRES-EGFP mice (Catalog Number: C001338), which conditionally express the human c-Myc oncogene, with Alb-Cre mice that express Cre recombinase specifically in hepatocytes under the control of the Alb promoter. The Cre-mediated recombination results in the deletion of the transcriptional stop sequence (Loxp-Stop-Loxp, LSL) in H11-CAG-LSL-hMYC-IRES-EGFP mice, leading to overexpression of the MYC oncogene in the liver and subsequent carcinogenesis. This model, therefore, spontaneously develops liver cancer with an early onset.
Strain Strategy
H11-CAG-LSL-hMYC-IRES-EGFP mice (Catalog Number: C001338) were constructed by integrating the CAG-LSL-hMYC-IRES-EGFP expression element into the H11 safe harbor locus of the mouse genome. By crossing these mice with liver-specific Alb-Cre mice, a model overexpressing the human MYC CDS in the liver, i.e., Alb-Cre+/MYC+ mice (Catalog Number: C001339), was generated.
Figure 1. Diagram of the gene editing strategy for the generation of Alb-cre+/MYC+ mice.
Application Area
<b>Liver Cancer Research:</b> The model overexpresses the MYC oncogene in the liver, spontaneously developing liver cancer, thus serving as a tool to study the mechanisms of liver cancer initiation and progression.
<b>Drug Screening and Evaluation: </b> Utilized to assess the efficacy of new anti-liver cancer drugs and therapeutic strategies, particularly those targeting MYC-driven tumors.
<b>Gene Function Studies: </b> Investigates the role of the MYC gene in hepatocytes and its involvement in tumor formation.
<b>Transcriptional Regulation Studies:</b> Explores the mechanisms of MYC in transcriptional regulation and its interactions with other genes and signaling pathways.
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