Calb1-dgCre (DHFR-EGFP-Cre)
Product ID:
C001747
Background:
C57BL/6JCya
Documents:
Expressing Tissues/Cells:
Hippocampus, striatum, thalamus, midbrain, cerebellum, and Calb1-positive cells
Product Type
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Genotype
Sex
Quantity
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Basic Information
Model Name
Calb1-dgCre (DHFR-EGFP-Cre)
Product ID
C001747
Gene Name
Background
C57BL/6JCya
Gene Alias
CB; Calb; Calb-1; Brain-2
Classification
Cre Driver Lines,Reporter Mouse Lines
NCBI ID
Phenotype
Chromosome
4
Document
Note: When using this mouse strain in a publication, please cite “Calb1-dgCre (DHFR-EGFP-Cre) mice (Catalog C001747) were purchased from Cyagen.”
Strain Description
The TAG stop codon was replaced with the T2A-dgCre (DHFR-EGFP-Cre) cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Calb1 gene elements, without impacting the endogenous expression of the Calb1 gene. The DHFR (dihydrofolate reductase) fragment, derived from the N-terminal 159 amino acids of E. coli ecDHFR, functions as a destabilizing domain (DD). In the absence of specific ligands like TMP (Trimethoprim), this fragment leads to the rapid proteasomal degradation of Cre recombinase. This mechanism allows for the temporal-specific induction of Cre recombinase activity by regulating DHFR-Cre stability with small molecules such as TMP. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.
Validation Data