The gene encoding lecithin:retinol acyltransferase (LRAT) produces an enzyme responsible for retinyl esterification within the endoplasmic reticulum. LRAT plays a crucial role in vitamin A metabolism within the visual system. Beyond its essential function in the visual cycle, LRAT is also critical for retinol metabolism in tissues such as the liver, testes, and small intestine. LRAT-positive cells are primarily found in the narrow gap between hepatocytes and sinusoidal endothelial cells known as the Disse space. Additionally, LRAT expression occurs in extraembryonic components, intestines, liver, midbrain, and reproductive organs. At the cellular level, LRAT is predominantly localized in the rough endoplasmic reticulum and multivesicular bodies of hepatic stellate cells (HSCs). The Lrat-P2A-tdTomato-T2A-CreERT2 mouse model, generated by integrating the P2A-tdTomato-T2A-CreERT2 expression cassette at the Lrat gene stop codon, allows inducible Cre recombinase expression driven by Lrat gene regulatory elements. The expression pattern of CreERT2 recombinase mirrors that of endogenous Lrat, without affecting endogenous Lrat expression in mice. When Lrat-P2A-tdTomato-T2A-CreERT2 mice are crossed with mice carrying loxP sites, Cre recombinase-mediated sequence recombination is expected to occur in the progeny’s hepatic stellate cells and other cells expressing Lrat.