C57BL/6JCya-Acacbem1flox/Cya
Common Name:
Acacb-flox
Product ID:
S-CKO-00168
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Acacb-flox
Strain ID
CKOCMP-100705-Acacb-B6J-VA
Gene Name
Product ID
S-CKO-00168
Gene Alias
Acc2; Accb
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acacbem1flox/Cya mice (Catalog S-CKO-00168) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031583
NCBI RefSeq
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Target Region
Exon 4~8
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Acacb, also known as acetyl-coenzyme A carboxylase β, is the rate-limiting enzyme in fatty acid oxidation [4]. It plays a crucial role in fatty acid metabolism pathways. Continuous fatty acid oxidation in Acacb knock-out mice increases insulin sensitivity, highlighting the importance of genetic models in studying its function [4].
In Caucasian diabetic patients, the rs2268388 polymorphism in the ACACB gene is associated with diabetic nephropathy, with the C allele showing a decreased susceptibility risk compared to the T allele [1]. In metastatic colorectal cancer, ACACB is a key metabolism-related biomarker, and its high expression can lead to decreased sensitivity to cetuximab, potentially through affecting EGFR phosphorylation and the mTOR/Akt signaling pathway [2]. In mice, cardiac-specific deletion of Acc2 (a form of Acacb) increases fatty acid oxidation, preventing high-fat diet-induced cardiomyopathy by regulating parkin-mediated mitophagy [3].
In conclusion, Acacb is essential in fatty acid oxidation and is involved in important biological processes and disease conditions. Studies using gene knockout mouse models have revealed its role in diabetes-related nephropathy, metastatic colorectal cancer, and diet-induced cardiomyopathy, contributing to our understanding of these diseases and potentially guiding future therapeutic strategies.
References:
1. An, Li, Jiang, Hao, Tang, Ri-Ning. 2015. The ACACB gene rs2268388 polymorphism is associated with nephropathy in Caucasian patients with diabetes: a meta-analysis. In Renal failure, 37, 925-8. doi:10.3109/0886022X.2015.1052978. https://pubmed.ncbi.nlm.nih.gov/26030797/
2. Hong, Hi-Ju, Shao, Yanfei, Zhang, Sen, Sun, Jing, Zheng, Minhua. . ACACB is a novel metabolism-related biomarker in the prediction of response to cetuximab therapy inmetastatic colorectal cancer. In Acta biochimica et biophysica Sinica, 54, 1671-1683. doi:10.3724/abbs.2022121. https://pubmed.ncbi.nlm.nih.gov/36111743/
3. Shao, Dan, Kolwicz, Stephen C, Wang, Pei, Regnier, Michael, Tian, Rong. 2020. Increasing Fatty Acid Oxidation Prevents High-Fat Diet-Induced Cardiomyopathy Through Regulating Parkin-Mediated Mitophagy. In Circulation, 142, 983-997. doi:10.1161/CIRCULATIONAHA.119.043319. https://pubmed.ncbi.nlm.nih.gov/32597196/
4. Ma, Lijun, Mondal, Ashis K, Murea, Mariana, Elbein, Steven C, Freedman, Barry I. 2011. The effect of ACACB cis-variants on gene expression and metabolic traits. In PloS one, 6, e23860. doi:10.1371/journal.pone.0023860. https://pubmed.ncbi.nlm.nih.gov/21887335/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen