C57BL/6NCya-Dyrk4em1flox/Cya
Common Name:
Dyrk4-flox
Product ID:
S-CKO-00204
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dyrk4-flox
Strain ID
CKOCMP-101320-Dyrk4-B6N-VA
Gene Name
Product ID
S-CKO-00204
Gene Alias
Dyrk4a; Dyrk4b
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Dyrk4em1flox/Cya mice (Catalog S-CKO-00204) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000078521
NCBI RefSeq
NM_207210
Target Region
Exon 4~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
DYRK4, a member of the dual-specificity tyrosine phosphorylation-regulated kinase family, plays important roles in multiple biological processes. It is involved in antiviral innate immunity, cell differentiation, proliferation, and survival. It has been associated with pathways related to interferon-β induction and may have a role in spermatogenesis as well [1,4,5]. Genetic models, such as knockout mice, can be valuable tools for studying its functions.
In antiviral innate immunity, DYRK4 acts as a scaffold protein. Overexpression of DYRK4 enhances virus-triggered activation of IRF3 and type I IFN induction, while knockdown or knockout of DYRK4 impairs virus-induced activation of IRF3 and NF-κB. Dyrk4-knockout mice are more susceptible to viral infection, suggesting its importance in antiviral defense [1]. In the context of cancer, hypomethylation of DYRK4 in peripheral blood is associated with an increased lung cancer risk. Also, a novel neoplastic fusion transcript, RAD51AP1-DYRK4, is preferentially expressed in luminal B breast tumors, endowing increased sensitivity to the MEK inhibitor trametinib [2,3].
In conclusion, DYRK4 is crucial for antiviral innate immunity and is associated with cancer-related processes. The use of Dyrk4-knockout mouse models has provided insights into its role in antiviral responses and potential implications in cancer. Understanding DYRK4 functions can contribute to developing strategies for treating viral infections and certain cancers.
References:
1. Zeng, Xianhuang, Xu, Jiaqi, Liu, Jiaqi, Guo, Mingxiong, Sun, Guihong. 2024. DYRK4 upregulates antiviral innate immunity by promoting IRF3 activation. In EMBO reports, 26, 690-719. doi:10.1038/s44319-024-00352-x. https://pubmed.ncbi.nlm.nih.gov/39702801/
2. Qiao, Rong, Zhu, Qiang, Di, Feifei, Han, Baohui, Yang, Rongxi. 2023. Hypomethylation of DYRK4 in peripheral blood is associated with increased lung cancer risk. In Molecular carcinogenesis, 62, 1745-1754. doi:10.1002/mc.23612. https://pubmed.ncbi.nlm.nih.gov/37530470/
3. Liu, Chia-Chia, Veeraraghavan, Jamunarani, Tan, Ying, Hu, Yiheng, Wang, Xiao-Song. 2020. A Novel Neoplastic Fusion Transcript, RAD51AP1-DYRK4, Confers Sensitivity to the MEK Inhibitor Trametinib in Aggressive Breast Cancers. In Clinical cancer research : an official journal of the American Association for Cancer Research, 27, 785-798. doi:10.1158/1078-0432.CCR-20-2769. https://pubmed.ncbi.nlm.nih.gov/33172895/
4. Sacher, F, Möller, C, Bone, W, Gottwald, U, Fritsch, M. 2007. The expression of the testis-specific Dyrk4 kinase is highly restricted to step 8 spermatids but is not required for male fertility in mice. In Molecular and cellular endocrinology, 267, 80-8. doi:. https://pubmed.ncbi.nlm.nih.gov/17292540/
5. Papadopoulos, Chrisovalantis, Arato, Krisztina, Lilienthal, Eva, Becker, Walter, de la Luna, Susana. 2010. Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. In The Journal of biological chemistry, 286, 5494-505. doi:10.1074/jbc.M110.157909. https://pubmed.ncbi.nlm.nih.gov/21127067/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen