C57BL/6JCya-Ano1em1flox/Cya
Common Name:
Ano1-flox
Product ID:
S-CKO-00235
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ano1-flox
Strain ID
CKOCMP-101772-Ano1-B6J-VA
Gene Name
Product ID
S-CKO-00235
Gene Alias
Tmem16a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ano1em1flox/Cya mice (Catalog S-CKO-00235) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000121758
NCBI RefSeq
NM_178642
Target Region
Exon 4~5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Ano1, also known as TMEM16A, encodes a member of Ca2+ -activated Cl- channels (CaCCs). These channels are crucial for physiological functions such as epithelial secretion, smooth muscle contraction, and sensory signal transduction [1]. ANO1 controls multiple cell functions including proliferation, survival, migration, and secretion [8].
ANO1's abnormal expression or dysfunction is involved in many pathological phenotypes and diseases. In cancer, it plays a vital role in the occurrence, development, metastasis, proliferation, and apoptosis of various malignant tumors [2]. Knocking-down or inhibiting ANO1 suppresses the growth, metastasis, and invasion of multiple gastrointestinal cancer cell lines and xenograft models, and enhances immunotherapeutic effectiveness [3]. In addition, over-expression of ANO1 is significantly correlated with poor overall survival in various cancers [5].
In the context of cystic fibrosis, pharmacological stimulation of TMEM16A (ANO1) could potentially bypass CFTR defect, but the relationship with mucus hypersecretion needs clarification [4]. In keratinocytes, ANO1-mediated chloride influx enhances wound healing [7]. In the urethra, the role of Ano1 channels in generating urethral tone is still debated due to differences in species and experimental approaches [6].
In conclusion, Ano1 is essential for multiple physiological functions and is significantly associated with various diseases, especially cancer. Studies using loss-of-function models like knocking-down or inhibiting ANO1 have revealed its role in promoting cancer progression and immunotherapeutic resistance, providing potential therapeutic targets for these diseases.
References:
1. Liu, Yani, Liu, Zongtao, Wang, KeWei. 2020. The Ca2+-activated chloride channel ANO1/TMEM16A: An emerging therapeutic target for epithelium-originated diseases? In Acta pharmaceutica Sinica. B, 11, 1412-1433. doi:10.1016/j.apsb.2020.12.003. https://pubmed.ncbi.nlm.nih.gov/34221860/
2. Guo, Saisai, Zhang, Linna, Li, Na. 2022. ANO1: More Than Just Calcium-Activated Chloride Channel in Cancer. In Frontiers in oncology, 12, 922838. doi:10.3389/fonc.2022.922838. https://pubmed.ncbi.nlm.nih.gov/35734591/
3. Jiang, Fangli, Jia, Keren, Chen, Yang, Shen, Lin, Zhang, Cheng. 2023. ANO1-Mediated Inhibition of Cancer Ferroptosis Confers Immunotherapeutic Resistance through Recruiting Cancer-Associated Fibroblasts. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2300881. doi:10.1002/advs.202300881. https://pubmed.ncbi.nlm.nih.gov/37341301/
4. Galietta, Luis J V. 2022. TMEM16A (ANO1) as a therapeutic target in cystic fibrosis. In Current opinion in pharmacology, 64, 102206. doi:10.1016/j.coph.2022.102206. https://pubmed.ncbi.nlm.nih.gov/35364521/
5. Zhang, Congxiao, Li, Haini, Gao, Jing, Yang, Shengmei, Liu, Zongtao. . Prognostic significance of ANO1 expression in cancers. In Medicine, 100, e24525. doi:10.1097/MD.0000000000024525. https://pubmed.ncbi.nlm.nih.gov/33530281/
6. Drumm, Bernard T, Thornbury, Keith D, Hollywood, Mark A, Sergeant, Gerard P. 2021. Role of Ano1 Ca2+-activated Cl- channels in generating urethral tone. In American journal of physiology. Renal physiology, 320, F525-F536. doi:10.1152/ajprenal.00520.2020. https://pubmed.ncbi.nlm.nih.gov/33554780/
7. Yamanoi, Yu, Lei, Jing, Takayama, Yasunori, Marunaka, Yoshinori, Tominaga, Makoto. 2023. TRPV3-ANO1 interaction positively regulates wound healing in keratinocytes. In Communications biology, 6, 88. doi:10.1038/s42003-023-04482-1. https://pubmed.ncbi.nlm.nih.gov/36690845/
8. Dulin, Nickolai O. 2020. Calcium-Activated Chloride Channel ANO1/TMEM16A: Regulation of Expression and Signaling. In Frontiers in physiology, 11, 590262. doi:10.3389/fphys.2020.590262. https://pubmed.ncbi.nlm.nih.gov/33250781/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen