C57BL/6JCya-Lars2em1flox/Cya
Common Name:
Lars2-flox
Product ID:
S-CKO-00267
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lars2-flox
Strain ID
CKOCMP-102436-Lars2-B6J-VA
Gene Name
Product ID
S-CKO-00267
Gene Alias
Kiaa0028; LEURS
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lars2em1flox/Cya mice (Catalog S-CKO-00267) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038863
NCBI RefSeq
NM_153168
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Lars2, an enzyme responsible for synthesizing mitochondrial leucyl-tRNA, is crucial for maintaining mitochondrial function. It is involved in pathways such as the phosphatidylinositol 3-kinase/protein kinase B (PI3K-AKT) pathway. Its proper function is of great biological importance as it impacts various cellular processes related to mitochondrial activity [1,3].
In Alzheimer's disease (AD) research, knockdown of Lars2 in mouse neuroblastoma cells (neuro-2a cells) and primary cortical neurons led to morphological changes and decreased density in mouse hippocampal neurons. In vivo, knockout of lars2 in hippocampal neurons caused cognitive impairment as shown by the Barnes maze test, the novel object recognition test, and nest-building experiments. Downregulated Lars2 expression impeded the PI3K-AKT pathway, activating GSK3β, which led to excessive phosphorylation of Tau protein and subsequent neuronal degeneration, suggesting Lars2 as a potential therapeutic target for AD [1].
In ovarian granulosa cells, silencing of Lars2 inhibited cell proliferation and promoted apoptosis, induced mitochondrial dysfunction and ROS accumulation, indicating its role in the etiology of premature ovarian insufficiency (POI) [2].
In conclusion, Lars2 is essential for mitochondrial function and is involved in multiple disease-related biological processes. Gene knockout models in mice have been instrumental in revealing its role in AD and POI, providing valuable insights into the underlying mechanisms of these diseases and potential therapeutic strategies related to Lars2.
References:
1. Qian, Wenqi, Yuan, Lin, Zhuge, Weishan, Ni, Haoqi, Lv, Xinhuang. 2024. Regulating Lars2 in mitochondria: A potential Alzheimer's therapy by inhibiting tau phosphorylation. In Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 21, e00353. doi:10.1016/j.neurot.2024.e00353. https://pubmed.ncbi.nlm.nih.gov/38575503/
2. Feng, Shujun, Wan, Shan, Liu, Shuangying, Bai, Long, Zhu, Yimin. 2022. LARS2 Regulates Apoptosis via ROS-Mediated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Ovarian Granulosa Cells. In Oxidative medicine and cellular longevity, 2022, 5501346. doi:10.1155/2022/5501346. https://pubmed.ncbi.nlm.nih.gov/35585880/
3. Li, Dingfeng, Gao, Xinyi, Ma, Xiaolin, Zhang, Juan, Liu, Qiang. 2024. Aging-induced tRNAGlu-derived fragment impairs glutamate biosynthesis by targeting mitochondrial translation-dependent cristae organization. In Cell metabolism, 36, 1059-1075.e9. doi:10.1016/j.cmet.2024.02.011. https://pubmed.ncbi.nlm.nih.gov/38458203/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen