C57BL/6JCya-Cd276em1flox/Cya
Common Name:
Cd276-flox
Product ID:
S-CKO-00295
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cd276-flox
Strain ID
CKOCMP-102657-Cd276-B6J-VA
Gene Name
Product ID
S-CKO-00295
Gene Alias
6030411F23Rik; B7-H3; B7RP-2; B7h3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cd276em1flox/Cya mice (Catalog S-CKO-00295) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165365
NCBI RefSeq
NM_133983
Target Region
Exon 3~4
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Cd276, also known as B7-H3, is an immunoregulatory protein belonging to the B7 family. It plays a key role in cancer progression and is involved in immune checkpoint regulation. Cd276 is selectively expressed in tumor cells and immune cells within the tumor microenvironment, participating in processes such as tumor cell proliferation, metastasis, and immune evasion, and is thus a promising target for cancer therapy [1,3,4].
In mouse models, genetic ablation of Cd276 in tumor-associated macrophages (TAMs) blocked efferocytosis and enhanced the expression of major histocompatibility complex class II (MHCII) of TAMs, increasing CD4+ and cytotoxic CD8+ T cell infiltration in bladder cancer [2]. In esophageal squamous cell carcinoma (ESCC), depletion of Cd276 in a 4-nitroquinoline 1-oxide (4NQO)-induced mouse model inhibited tumorigenesis and progression. Conditional knockout of Cd276 in epithelial cells downregulated CXCL1, reducing the formation of neutrophil extracellular trap networks (NETs) via the CXCL1-CXCR2 signaling axis and augmenting natural killer (NK) cells [5].
In conclusion, Cd276 is significantly involved in tumor immune evasion, proliferation, and metastasis. Gene knockout and conditional knockout mouse models have revealed its role in bladder cancer and ESCC, highlighting its potential as a therapeutic target in these cancer types. Understanding Cd276's functions through these research models provides new insights for developing cancer immunotherapies.
References:
1. Zhou, Wu-Tong, Jin, Wei-Lin. 2021. B7-H3/CD276: An Emerging Cancer Immunotherapy. In Frontiers in immunology, 12, 701006. doi:10.3389/fimmu.2021.701006. https://pubmed.ncbi.nlm.nih.gov/34349762/
2. Cheng, Maosheng, Chen, Shuang, Li, Kang, Li, Yang, Peng, Liang. 2024. CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer. In Nature communications, 15, 2818. doi:10.1038/s41467-024-46735-5. https://pubmed.ncbi.nlm.nih.gov/38561369/
3. Getu, Ayechew Adera, Tigabu, Abiye, Zhou, Ming, Fodstad, Øystein, Tan, Ming. 2023. New frontiers in immune checkpoint B7-H3 (CD276) research and drug development. In Molecular cancer, 22, 43. doi:10.1186/s12943-023-01751-9. https://pubmed.ncbi.nlm.nih.gov/36859240/
4. Liu, Shengzhuo, Liang, Jiayu, Liu, Zhihong, Lu, Yiping, Wang, Xianding. 2021. The Role of CD276 in Cancers. In Frontiers in oncology, 11, 654684. doi:10.3389/fonc.2021.654684. https://pubmed.ncbi.nlm.nih.gov/33842369/
5. Xiong, Gan, Chen, Zhi, Liu, Qianwen, Chen, Demeng, Zhou, Qimin. 2024. CD276 regulates the immune escape of esophageal squamous cell carcinoma through CXCL1-CXCR2 induced NETs. In Journal for immunotherapy of cancer, 12, . doi:10.1136/jitc-2023-008662. https://pubmed.ncbi.nlm.nih.gov/38724465/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen