C57BL/6JCya-Tmed4em1flox/Cya
Common Name:
Tmed4-flox
Product ID:
S-CKO-00332
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmed4-flox
Strain ID
CKOCMP-103694-Tmed4-B6J-VA
Gene Name
Product ID
S-CKO-00332
Gene Alias
1110014L17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed4em1flox/Cya mice (Catalog S-CKO-00332) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000004508
NCBI RefSeq
NM_134020
Target Region
Exon 1~4
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Tmed4, also known as endoplasmic reticulum stress-response protein 25 (ERS25), is a member of the transmembrane p24 trafficking protein family. It is involved in protein vesicular trafficking between the endoplasmic reticulum (ER) and Golgi compartments, and is associated with endoplasmic reticulum stress (ERS) and the nuclear factor erythroid 2-related factor 2-related (NRF2-related) antioxidant response pathways [1,3]. It has significance in maintaining cellular homeostasis and is potentially important in various biological processes and diseases [1,2]. Genetic models, such as gene knockout mouse models, are valuable for studying its functions.
In Treg-specific KO (Tmed4ΔTreg) mice, loss of Tmed4 led to defects in ERS and the NRF2-related antioxidant response, resulting in excessive reactive oxygen species (ROS). This reduced the Foxp3 stability and suppressive function of regulatory T cells (Tregs) in an IRE1α/XBP1 axis-dependent manner, causing T cell hyperactivation, an exacerbated inflammatory phenotype, and boosted antitumor immunity [1].
In conclusion, Tmed4 is crucial for maintaining the stability of Tregs and their suppressive function through the IRE1α-dependent ROS and the NRF2-related antioxidant response. The Tmed4ΔTreg mouse model has provided insights into the role of Tmed4 in tumor and autoimmune disease conditions, highlighting its potential as a therapeutic target in these areas [1].
References:
1. Jiang, Zhenyan, Wang, Huizi, Wang, Xiaoxia, Li, Bin, Wu, Xuefeng. 2024. TMED4 facilitates regulatory T cell suppressive function via ROS homeostasis in tumor and autoimmune mouse models. In The Journal of clinical investigation, 135, . doi:10.1172/JCI179874. https://pubmed.ncbi.nlm.nih.gov/39480507/
2. Huang, Shuwen, Li, Yong, Shen, Jianying, Liang, Wenna, Li, Candong. 2023. Identification of a diagnostic model and molecular subtypes of major depressive disorder based on endoplasmic reticulum stress-related genes. In Frontiers in psychiatry, 14, 1168516. doi:10.3389/fpsyt.2023.1168516. https://pubmed.ncbi.nlm.nih.gov/37649561/
3. Hwang, Sun Ok, Boswell, Sarah A, Seo, Jeong-Sun, Lee, Sam W. 2008. Novel oxidative stress-responsive gene ERS25 functions as a regulator of the heat-shock and cell death response. In The Journal of biological chemistry, 283, 13063-9. doi:10.1074/jbc.M709656200. https://pubmed.ncbi.nlm.nih.gov/18326488/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen