C57BL/6JCya-Ces1dem1flox/Cya
Common Name:
Ces1d-flox
Product ID:
S-CKO-00370
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ces1d-flox
Strain ID
CKOCMP-104158-Ces1d-B6J-VA
Gene Name
Product ID
S-CKO-00370
Gene Alias
Ces3; TGH
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ces1dem1flox/Cya mice (Catalog S-CKO-00370) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034172
NCBI RefSeq
NM_053200
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Ces1d, also known as carboxylesterase 1d, is a crucial enzyme belonging to the serine hydrolase superfamily. It has a wide range of activities, mainly localizing in the endoplasmic reticulum (ER) [2]. It is involved in metabolizing drugs, pesticides, and lipids, and has been implicated in hepatic triacylglycerol metabolism [3].
In gene knockout studies, Ces1d -deficient mice were protected from high-sucrose diet-induced hepatic lipid accumulation. Mechanistically, Ces1d deficiency led to the activation of AMP-activated protein kinase and inhibitory phosphorylation of acetyl-CoA carboxylase [1]. Adipose tissue-specific Ces1d knockout (FKO) mice showed metabolic dysregulation, including weight gain, impaired glucose and lipid metabolism, and exacerbated liver steatosis [2]. Liver-specific Ces1d-deficient mice did not show any difference in the flux of in vivo HDL-to-feces reverse cholesterol transport nor additional liver CE accumulation after a high-cholesterol diet challenge, challenging its role as a major hepatic cholesteryl ester hydrolase [3]. Deletion of the Ces1d gene in mice markedly reduced the acute ethanol-induced increase of blood fatty acid ethyl esters (FAEEs) levels with a slight but significant reduction of serum aminotransferase levels [4]. Ces1d -/- mice also exhibited augmented lung inflammation in response to lipopolysaccharide (LPS) [5]. Myeloid-specific Ces1d knockout mice had lipid dysregulation in macrophages, triggering pro-inflammatory activation and contributing to worsened metabolic disorders [6].
In conclusion, Ces1d plays a significant role in lipid metabolism, with its deficiency affecting various physiological processes. Gene knockout mouse models have been instrumental in revealing its role in non-alcoholic fatty liver disease (NAFLD), metabolic dysregulation, acute liver injury, and lung inflammation, providing potential pharmacological targets for these disease areas.
References:
1. Lian, Jihong, Watts, Russell, Quiroga, Ariel D, Alexander, R Todd, Lehner, Richard. 2019. Ces1d deficiency protects against high-sucrose diet-induced hepatic triacylglycerol accumulation. In Journal of lipid research, 60, 880-891. doi:10.1194/jlr.M092544. https://pubmed.ncbi.nlm.nih.gov/30737251/
2. Li, Gang, Li, Xin, Yang, Li, Lehner, Richard, Sun, Kai. 2022. Adipose tissue-specific ablation of Ces1d causes metabolic dysregulation in mice. In Life science alliance, 5, . doi:10.26508/lsa.202101209. https://pubmed.ncbi.nlm.nih.gov/35459739/
3. Lian, Jihong, van der Veen, Jelske N, Watts, Russell, Jacobs, René L, Lehner, Richard. 2021. Carboxylesterase 1d (Ces1d) does not contribute to cholesteryl ester hydrolysis in the liver. In Journal of lipid research, 62, 100093. doi:10.1016/j.jlr.2021.100093. https://pubmed.ncbi.nlm.nih.gov/34153284/
4. Park, Seol Hee, Seo, Wonhyo, Xu, Ming-Jiang, Liangpunsakul, Suthat, Gao, Bin. 2022. Ethanol and its Nonoxidative Metabolites Promote Acute Liver Injury by Inducing ER Stress, Adipocyte Death, and Lipolysis. In Cellular and molecular gastroenterology and hepatology, 15, 281-306. doi:10.1016/j.jcmgh.2022.10.002. https://pubmed.ncbi.nlm.nih.gov/36243320/
5. Szafran, Brittany N, Borazjani, Abdolsamad, Scheaffer, Hannah L, Kaplan, Barbara L F, Ross, Matthew K. 2022. Carboxylesterase 1d Inactivation Augments Lung Inflammation in Mice. In ACS pharmacology & translational science, 5, 919-931. doi:10.1021/acsptsci.2c00098. https://pubmed.ncbi.nlm.nih.gov/36268116/
6. Shao, Long J, Elizondo, Fathima, Gao, Feng, Wu, Huaizhu, Sun, Kai. 2025. Functional Regulation of Macrophages by Ces1d-Mediated Lipid Signaling in Immunometabolism. In Molecular metabolism, , 102166. doi:10.1016/j.molmet.2025.102166. https://pubmed.ncbi.nlm.nih.gov/40349771/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen