C57BL/6JCya-Npffr2em1flox/Cya
Common Name
Npffr2-flox
Product ID
S-CKO-00393
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-104443-Npffr2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Npffr2-flox Mouse (Catalog S-CKO-00393) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Npffr2-flox
Strain ID
CKOCMP-104443-Npffr2-B6J-VA
Gene Name
Product ID
S-CKO-00393
Gene Alias
HG31, Gpr74, NPFF2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000048557
NCBI RefSeq
NM_133192
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Overview of Gene Research
Npffr2, the neuropeptide FF receptor 2, is a G protein-coupled receptor (GPCR) that helps regulate pain, modulates the opioid system, and is involved in controlling energy balance, thermogenesis, and the hypothalamic-pituitary-adrenal (HPA) axis [2,4,5,6]. It may also be associated with the RhoA/YAP signaling pathway in cancer [2]. Genetic models, such as knockout mice, are valuable for studying its functions.
In NPFFR2-deficient mice, several phenotypes were observed. Those fed a high-fat diet developed severe glucose intolerance, along with hypothalamic insulin resistance, due to reduced insulin pathway signaling proteins [1]. In hepatocellular carcinoma, silencing of NPFFR2 decreased cancer cell malignancy, while overexpression increased invasion, migration, and anchorage-independent cell growth, suggesting its role in promoting cancer progression [2]. NPFFR2-knockout mice also showed resistance to LPS-induced depressive-like behaviors and stress-exposure-induced anxiety-like behaviors and HPA axis hyperactivity [3,4].
In conclusion, Npffr2 plays essential roles in metabolism, cancer malignancy, stress-related disorders, and pain modulation. Studies using NPFFR2-knockout mouse models have provided insights into its functions in these disease-related processes, offering potential targets for treating conditions such as diabetes, hepatocellular carcinoma, depression, and anxiety-related disorders.
References：
1. Karnošová, Alena, Strnadová, Veronika, Železná, Blanka, Kašpárek, Petr, Maletínská, Lenka. . NPFFR2-deficient mice fed a high-fat diet develop strong intolerance to glucose. In Clinical science (London, England : 1979), 137, 847-862. doi:10.1042/CS20220880. https://pubmed.ncbi.nlm.nih.gov/37191311/
2. Shin, Yuna, Jung, Wonhee, Kim, Mi-Yeon, Kim, Sang-Bum, Shin, Hyun Jin. 2022. NPFFR2 Contributes to the Malignancy of Hepatocellular Carcinoma Development by Activating RhoA/YAP Signaling. In Cancers, 14, . doi:10.3390/cancers14235850. https://pubmed.ncbi.nlm.nih.gov/36497331/
3. Yu, Zachary, Lin, Ya-Tin, Chen, Jin-Chung. 2021. Knockout of NPFFR2 Prevents LPS-Induced Depressive-Like Responses in Mice. In International journal of molecular sciences, 22, . doi:10.3390/ijms22147611. https://pubmed.ncbi.nlm.nih.gov/34299230/
4. Lin, Ya-Tin, Huang, Yi-Ling, Tsai, Sze-Chi, Chen, Jin-Chung. 2020. Ablation of NPFFR2 in Mice Reduces Response to Single Prolonged Stress Model. In Cells, 9, . doi:10.3390/cells9112479. https://pubmed.ncbi.nlm.nih.gov/33202667/
5. Lin, Ya-Tin, Yu, Yu-Lian, Hong, Wei-Chen, Chen, Ting-Chun, Chen, Jin-Chung. 2017. NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in Rodents. In International journal of molecular sciences, 18, . doi:10.3390/ijms18081810. https://pubmed.ncbi.nlm.nih.gov/28825666/
6. Lin, Ya-Tin, Wu, Kuan-Hsuan, Jhang, Jie-Jhu, Hsu, Po-Hung, Li, Hui-Yun. 2024. Hypothalamic NPFFR2 attenuates central insulin signaling and its knockout diminishes metabolic dysfunction in mouse models of diabetes mellitus. In Clinical nutrition (Edinburgh, Scotland), 43, 603-619. doi:10.1016/j.clnu.2024.01.013. https://pubmed.ncbi.nlm.nih.gov/38301284/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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