C57BL/6NCya-Ddx1em1flox/Cya
Common Name
Ddx1-flox
Product ID
S-CKO-00405
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-104721-Ddx1-B6N-VA
Status
When using this mouse strain in a publication, please cite “Ddx1-flox Mouse (Catalog S-CKO-00405) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ddx1-flox
Strain ID
CKOCMP-104721-Ddx1-B6N-VA
Gene Name
Product ID
S-CKO-00405
Gene Alias
DBP-RB
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 12
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000071103
NCBI RefSeq
NM_134040
Target Region
Exon 4~5
Size of Effective Region
~2.2 kb
Overview of Gene Research
Ddx1, a member of the DEAD box family of RNA helicases, is involved in multiple aspects of RNA metabolism, such as 3'-end RNA processing, DNA repair, microRNA processing, tRNA maturation, and mRNA transport [2,6]. It has also been linked to R-loop formation and resolution, and is associated with pathways like DNA repair, RNA transport, and replication [4,5].
In mouse models, Ddx1 knockout leads to embryo stalling at the 2-4 cell stages, disruption of calcium distribution, increased mitochondria membrane potential, mitochondrial activity, and reactive oxygen species [1]. Additionally, Ddx1 knockout results in transgenerational wild-type lethality in mice [6]. In non-small cell lung cancer cells, depleting Ddx1 increases sensitivity to chemotherapy by attenuating cancer stem cell traits [3].
In conclusion, Ddx1 is essential for embryonic development, and its function is crucial in maintaining normal cellular processes. Mouse knockout models have revealed its significant roles in embryonic development and cancer-related processes, highlighting its potential as a therapeutic target in cancer treatment.
References：
1. Wang, Yixiong, Yasmin, Lubna, Li, Lei, Sun, Xuejun, Godbout, Roseline. 2022. DDX1 vesicles control calcium-dependent mitochondrial activity in mouse embryos. In Nature communications, 13, 3794. doi:10.1038/s41467-022-31497-9. https://pubmed.ncbi.nlm.nih.gov/35778392/
2. Cargill, Michael, Venkataraman, Rasika, Lee, Stanley. 2021. DEAD-Box RNA Helicases and Genome Stability. In Genes, 12, . doi:10.3390/genes12101471. https://pubmed.ncbi.nlm.nih.gov/34680866/
3. Yang, Qi, Xu, Pei, Liu, Qingtao, Ding, Fangbao, Xiao, Haibo. 2023. Depleting DDX1 sensitizes non-small cell lung cancer cells to chemotherapy by attenuating cancer stem cell traits. In Life sciences, 323, 121592. doi:10.1016/j.lfs.2023.121592. https://pubmed.ncbi.nlm.nih.gov/36934972/
4. Gao, Baocai, Li, Xiangnan, Li, Shujie, Wu, Jiaxue, Li, Jixi. 2022. Pan-cancer analysis identifies RNA helicase DDX1 as a prognostic marker. In Phenomics (Cham, Switzerland), 2, 33-49. doi:10.1007/s43657-021-00034-x. https://pubmed.ncbi.nlm.nih.gov/36939765/
5. Yang, Shizhuo, Winstone, Lacey, Mondal, Sohaumn, Wu, Yuliang. 2023. Helicases in R-loop Formation and Resolution. In The Journal of biological chemistry, 299, 105307. doi:10.1016/j.jbc.2023.105307. https://pubmed.ncbi.nlm.nih.gov/37778731/
6. Hildebrandt, Matthew R, Germain, Devon R, Monckton, Elizabeth A, Brun, Miranda, Godbout, Roseline. 2015. Ddx1 knockout results in transgenerational wild-type lethality in mice. In Scientific reports, 5, 9829. doi:10.1038/srep09829. https://pubmed.ncbi.nlm.nih.gov/25909345/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.