C57BL/6NCya-Ddx1em1flox/Cya
Common Name:
Ddx1-flox
Product ID:
S-CKO-00405
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ddx1-flox
Strain ID
CKOCMP-104721-Ddx1-B6N-VA
Gene Name
Product ID
S-CKO-00405
Gene Alias
DBP-RB
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ddx1em1flox/Cya mice (Catalog S-CKO-00405) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071103
NCBI RefSeq
NM_134040
Target Region
Exon 4~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Ddx1, a member of the DEAD box family of RNA helicases, is involved in multiple aspects of RNA metabolism, such as 3'-end RNA processing, DNA repair, microRNA processing, tRNA maturation, and mRNA transport [2,6]. It has also been linked to R-loop formation and resolution, and is associated with pathways like DNA repair, RNA transport, and replication [4,5].
In mouse models, Ddx1 knockout leads to embryo stalling at the 2-4 cell stages, disruption of calcium distribution, increased mitochondria membrane potential, mitochondrial activity, and reactive oxygen species [1]. Additionally, Ddx1 knockout results in transgenerational wild-type lethality in mice [6]. In non-small cell lung cancer cells, depleting Ddx1 increases sensitivity to chemotherapy by attenuating cancer stem cell traits [3].
In conclusion, Ddx1 is essential for embryonic development, and its function is crucial in maintaining normal cellular processes. Mouse knockout models have revealed its significant roles in embryonic development and cancer-related processes, highlighting its potential as a therapeutic target in cancer treatment.
References：
1. Wang, Yixiong, Yasmin, Lubna, Li, Lei, Sun, Xuejun, Godbout, Roseline. 2022. DDX1 vesicles control calcium-dependent mitochondrial activity in mouse embryos. In Nature communications, 13, 3794. doi:10.1038/s41467-022-31497-9. https://pubmed.ncbi.nlm.nih.gov/35778392/
2. Cargill, Michael, Venkataraman, Rasika, Lee, Stanley. 2021. DEAD-Box RNA Helicases and Genome Stability. In Genes, 12, . doi:10.3390/genes12101471. https://pubmed.ncbi.nlm.nih.gov/34680866/
3. Yang, Qi, Xu, Pei, Liu, Qingtao, Ding, Fangbao, Xiao, Haibo. 2023. Depleting DDX1 sensitizes non-small cell lung cancer cells to chemotherapy by attenuating cancer stem cell traits. In Life sciences, 323, 121592. doi:10.1016/j.lfs.2023.121592. https://pubmed.ncbi.nlm.nih.gov/36934972/
4. Gao, Baocai, Li, Xiangnan, Li, Shujie, Wu, Jiaxue, Li, Jixi. 2022. Pan-cancer analysis identifies RNA helicase DDX1 as a prognostic marker. In Phenomics (Cham, Switzerland), 2, 33-49. doi:10.1007/s43657-021-00034-x. https://pubmed.ncbi.nlm.nih.gov/36939765/
5. Yang, Shizhuo, Winstone, Lacey, Mondal, Sohaumn, Wu, Yuliang. 2023. Helicases in R-loop Formation and Resolution. In The Journal of biological chemistry, 299, 105307. doi:10.1016/j.jbc.2023.105307. https://pubmed.ncbi.nlm.nih.gov/37778731/
6. Hildebrandt, Matthew R, Germain, Devon R, Monckton, Elizabeth A, Brun, Miranda, Godbout, Roseline. 2015. Ddx1 knockout results in transgenerational wild-type lethality in mice. In Scientific reports, 5, 9829. doi:10.1038/srep09829. https://pubmed.ncbi.nlm.nih.gov/25909345/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen