C57BL/6JCya-Peloem1flox/Cya
Common Name:
Pelo-flox
Product ID:
S-CKO-00427
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Pelo-flox
Strain ID
CKOCMP-105083-Pelo-B6J-VA
Gene Name
Product ID
S-CKO-00427
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Peloem1flox/Cya mice (Catalog S-CKO-00427) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109226
NCBI RefSeq
NM_134058
Target Region
Exon 1~2
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Pelo, a ribosome rescue factor, is involved in multiple biological processes. It plays a crucial role in cell meiotic division and mice embryonic development. It is also associated with translational quality control pathways [1,2].
Knockdown of PELO increased hemin-induced erythroid differentiation of K562 and HEL cells, inhibited cell proliferation and cell cycle progression, and promoted apoptosis of K562 cells. PELO regulates erythroid differentiation by interacting with MYC to upregulate KLF10 [1]. PELO also catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity [2]. In prostate cancer, PELO facilitates PLK1-induced the ubiquitination and degradation of Smad4, promoting the progression of prostate cancer [3]. In Drosophila, pelo deficiency limits the high-level synthesis of the Drosophila C virus capsid proteins, and in Aedes aegypti, pelo is upregulated during DENV replication and its silencing leads to reduced DENV virion production [4,5].
In conclusion, Pelo has diverse functions in biological processes such as erythroid differentiation, innate immune response, and viral replication. In disease-related contexts, it is involved in the progression of prostate cancer and the replication of certain viruses. Studies on Pelo, especially through loss-of-function experiments, help to understand its role in these biological processes and diseases, providing potential targets for therapeutic strategies.
References:
1. Hao, Jinglan, Han, Guiqin, Liang, Xin, Gao, Ping, Dong, Xiaoming. 2024. PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10. In The FEBS journal, 291, 4714-4731. doi:10.1111/febs.17254. https://pubmed.ncbi.nlm.nih.gov/39206622/
2. Wu, Xiurong, Yang, Zhang-Hua, Wu, Jianfeng, Han, Jiahuai. 2023. Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity. In Immunity, 56, 926-943.e7. doi:10.1016/j.immuni.2023.02.014. https://pubmed.ncbi.nlm.nih.gov/36948192/
3. Gao, Ping, Hao, Jing-Lan, Xie, Qian-Wen, Yan, Jian, Dong, Xiao-Ming. 2022. PELO facilitates PLK1-induced the ubiquitination and degradation of Smad4 and promotes the progression of prostate cancer. In Oncogene, 41, 2945-2957. doi:10.1038/s41388-022-02316-8. https://pubmed.ncbi.nlm.nih.gov/35437307/
4. Wu, Xiurong, He, Wan-Ting, Tian, Shuye, Chen, Jianming, Han, Jiahuai. 2014. pelo is required for high efficiency viral replication. In PLoS pathogens, 10, e1004034. doi:10.1371/journal.ppat.1004034. https://pubmed.ncbi.nlm.nih.gov/24722736/
5. Asad, Sultan, Hussain, Mazhar, Hugo, Leon, Watterson, Daniel, Asgari, Sassan. 2018. Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti. In PLoS neglected tropical diseases, 12, e0006405. doi:10.1371/journal.pntd.0006405. https://pubmed.ncbi.nlm.nih.gov/29641562/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen