C57BL/6JCya-Brd9em1flox/Cya
Common Name:
Brd9-flox
Product ID:
S-CKO-00437
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Brd9-flox
Strain ID
CKOCMP-105246-Brd9-B6J-VA
Gene Name
Product ID
S-CKO-00437
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Brd9em1flox/Cya mice (Catalog S-CKO-00437) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099384
NCBI RefSeq
NM_001024508
Target Region
Exon 4~5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Brd9, or Bromodomain-containing protein 9, is a subunit of the non-canonical BAF chromatin remodeling complex and a member of the bromodomain family IV. It plays a role in regulating gene transcription through epigenetic modification, recruiting the ncBAF complex to the promoter. Brd9 is involved in multiple biological pathways, such as the TGF-β/Activin/Nodal pathway, interferon-β signaling, and ribosome biogenesis, and is of great biological importance in processes like cell fate determination, self-renewal, and differentiation [4,5,6,8]. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
In myeloid lineage-specific Brd9-deficient mice, osteoclast lineage commitment and bone resorption were enhanced due to down-regulation of interferon-β signaling, revealing Brd9's role in suppressing osteoclastogenesis [1]. In Brd9-deficient hematopoietic stem cells, chromatin accessibility was increased, leading to myeloid lineage skewing and impaired B cell development, indicating its role in cell fate determination [2]. In pancreatic ductal adenocarcinoma, inhibition and genetic ablation of Brd9 in pancreatic cancer stem-like cells blocked self-renewal, invasiveness, and improved gemcitabine sensitivity [3]. In multiple myeloma, depleting Brd9 by genetic and pharmacologic approaches downregulated ribosome biogenesis genes, inhibited cell growth [6]. In HIF2αlow/− clear cell renal cell carcinoma, BRD9 knockdown or use of a selective antagonist suppressed cell growth [7]. In macrophages, inhibition of Brd9 led to reduction in interferon-stimulated genes [8].
In conclusion, Brd9 is crucial for regulating gene expression and participating in various biological processes. Model-based research, especially KO/CKO mouse models, has revealed its roles in bone diseases, hematological malignancies, pancreatic cancer, and other disease areas. Understanding Brd9's functions provides potential therapeutic targets for these diseases.
References:
1. Du, Jiahui, Liu, Yili, Wu, Xiaolin, Zhou, Mingliang, Jiang, Xinquan. 2023. BRD9-mediated chromatin remodeling suppresses osteoclastogenesis through negative feedback mechanism. In Nature communications, 14, 1413. doi:10.1038/s41467-023-37116-5. https://pubmed.ncbi.nlm.nih.gov/36918560/
2. Xiao, Muran, Kondo, Shinji, Nomura, Masaki, Abdel-Wahab, Omar, Inoue, Daichi. 2023. BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state. In Nature communications, 14, 8372. doi:10.1038/s41467-023-44081-6. https://pubmed.ncbi.nlm.nih.gov/38102116/
3. Feng, Yuliang, Cai, Liuyang, Pook, Martin, Jiang, Lei, Pauklin, Siim. 2023. BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma. In Gastroenterology, 166, 139-154. doi:10.1053/j.gastro.2023.09.021. https://pubmed.ncbi.nlm.nih.gov/37739089/
4. Zhu, Xiuzuo, Liao, Yi, Tang, Liling. 2020. Targeting BRD9 for Cancer Treatment: A New Strategy. In OncoTargets and therapy, 13, 13191-13200. doi:10.2147/OTT.S286867. https://pubmed.ncbi.nlm.nih.gov/33380808/
5. Wang, Xuepeng, Song, Chengcheng, Ye, Ying, Chen, Guokai, Zhang, Wensheng. . BRD9-mediated control of the TGF-β/Activin/Nodal pathway regulates self-renewal and differentiation of human embryonic stem cells and progression of cancer cells. In Nucleic acids research, 51, 11634-11651. doi:10.1093/nar/gkad907. https://pubmed.ncbi.nlm.nih.gov/37870468/
6. Kurata, Keiji, Samur, Mehmet K, Liow, Priscilla, Hideshima, Teru, Anderson, Kenneth C. . BRD9 Degradation Disrupts Ribosome Biogenesis in Multiple Myeloma. In Clinical cancer research : an official journal of the American Association for Cancer Research, 29, 1807-1821. doi:10.1158/1078-0432.CCR-22-3668. https://pubmed.ncbi.nlm.nih.gov/36780189/
7. Zhang, ChuanJie, Chen, Lu, Lou, WeiJuan, Xu, DanFeng, Li, QingQuan. 2021. Aberrant activation of m6A demethylase FTO renders HIF2αlow/- clear cell renal cell carcinoma sensitive to BRD9 inhibitors. In Science translational medicine, 13, eabf6045. doi:10.1126/scitranslmed.abf6045. https://pubmed.ncbi.nlm.nih.gov/34586831/
8. Ahmed, Nasiha S, Gatchalian, Jovylyn, Ho, Josephine, Evans, Ronald M, Hargreaves, Diana C. . BRD9 regulates interferon-stimulated genes during macrophage activation via cooperation with BET protein BRD4. In Proceedings of the National Academy of Sciences of the United States of America, 119, . doi:10.1073/pnas.2110812119. https://pubmed.ncbi.nlm.nih.gov/34983841/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen