C57BL/6NCya-Tnfaip8em1flox/Cya
Common Name:
Tnfaip8-flox
Product ID:
S-CKO-00530
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tnfaip8-flox
Strain ID
CKOCMP-106869-Tnfaip8-B6N-VA
Gene Name
Product ID
S-CKO-00530
Gene Alias
E130304C20Rik; Gg2-1; Gm10539; Nded; Ssc-2; Tipe
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tnfaip8em1flox/Cya mice (Catalog S-CKO-00530) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000148159
NCBI RefSeq
NM_134131
Target Region
Exon 2
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Tnfaip8, also known as Tumour necrosis factor (TNF)-α-inducible protein 8, is the founding member of the TIPE family. It acts as an anti-apoptotic protein, influencing multiple biological processes such as cell proliferation, metastasis, invasion, angiogenesis, drug resistance, and immune response through multiple signalling pathways like PI3K-AKT, mTOR-Akt-ULK1, and autophagy signals. It is of great importance in maintaining immune homeostasis and is associated with the development of various tumours and inflammatory diseases. Genetic models can help in further understanding its role [1,3,4,6,7,8,9].
In a streptozotocin-induced mouse model of diabetic retinopathy, overexpression of Tnfaip8 using an adeno-associated virus vector led to decreased a/b-wave amplitude, reduced retinal thickness, aggravated pathological abnormalities, increased avascular area, leukostasis, and up-regulated inflammatory factors in the retina, indicating its role in the pathogenesis of diabetic retinopathy [2]. In the context of kidney fibrosis, in vivo gain-or loss-of Tnfaip8 in a model of obstructive nephropathy either prevented or aggravated renal fibroblast apoptosis. Exosomal-Tnfaip8 promoted p53 ubiquitination and degradation, inhibiting fibroblast apoptosis and inducing proliferation [5].
In conclusion, Tnfaip8 plays crucial roles in maintaining immune homeostasis, tumour development, and inflammatory diseases. Studies using mouse models have revealed its functions in diabetic retinopathy and kidney fibrosis. Understanding Tnfaip8's functions can potentially offer novel therapeutic strategies for these and other related diseases.
References:
1. Hua, Jianyu, Zhuang, Guohong, Qi, Zhongquan. 2021. Current research status of TNFAIP8 in tumours and other inflammatory conditions (Review). In International journal of oncology, 59, . doi:10.3892/ijo.2021.5226. https://pubmed.ncbi.nlm.nih.gov/34036374/
2. Yang, Fuhua, Zhang, Hui, Yu, Xinyue, Zhang, Xiaomin, Li, Xiaorong. 2023. TNFAIP8 overexpression aggravates retinal pathophysiological features of diabetic retinopathy. In Experimental eye research, 234, 109572. doi:10.1016/j.exer.2023.109572. https://pubmed.ncbi.nlm.nih.gov/37451566/
3. Niture, Suryakant, Moore, John, Kumar, Deepak. . TNFAIP8: Inflammation, Immunity and Human Diseases. In Journal of cellular immunology, 1, 29-34. doi:. https://pubmed.ncbi.nlm.nih.gov/31723944/
4. Niture, Suryakant, Lin, Minghui, Odera, Joab O, Collins, Sean P, Kumar, Deepak. 2020. TNFAIP8 drives metabolic reprogramming to promote prostate cancer cell proliferation. In The international journal of biochemistry & cell biology, 130, 105885. doi:10.1016/j.biocel.2020.105885. https://pubmed.ncbi.nlm.nih.gov/33227392/
5. Liu, Xi, Liu, Zhao, Wang, Cong, Zhou, Lili, Liu, Youhua. 2023. Kidney tubular epithelial cells control interstitial fibroblast fate by releasing TNFAIP8-encapsulated exosomes. In Cell death & disease, 14, 672. doi:10.1038/s41419-023-06209-w. https://pubmed.ncbi.nlm.nih.gov/37828075/
6. Niture, Suryakant, Dong, Xialan, Arthur, Elena, Zheng, Weifan, Kumar, Deepak. 2018. Oncogenic Role of Tumor Necrosis Factor α-Induced Protein 8 (TNFAIP8). In Cells, 8, . doi:10.3390/cells8010009. https://pubmed.ncbi.nlm.nih.gov/30586922/
7. Lou, Yunwei, Liu, Suxia. 2011. The TIPE (TNFAIP8) family in inflammation, immunity, and cancer. In Molecular immunology, 49, 4-7. doi:10.1016/j.molimm.2011.08.006. https://pubmed.ncbi.nlm.nih.gov/21924498/
8. Niture, Suryakant, Gyamfi, Maxwell A, Lin, Minghui, Moore, John, Kumar, Deepak. 2020. TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation. In Cell death & disease, 11, 178. doi:10.1038/s41419-020-2369-4. https://pubmed.ncbi.nlm.nih.gov/32152268/
9. Chen, Zheng, Zhang, Jianguo, Dong, Chenyang, Yu, Wenhai, Chen, Yuezhi. 2021. TNFAIP8 regulates gastric cancer growth via mTOR-Akt-ULK1 pathway and autophagy signals. In Journal of cellular and molecular medicine, 25, 3361-3370. doi:10.1111/jcmm.16413. https://pubmed.ncbi.nlm.nih.gov/33682317/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen