C57BL/6JCya-Glyatem1flox/Cya
Common Name:
Glyat-flox
Product ID:
S-CKO-00547
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Glyat-flox
Strain ID
CKOCMP-107146-Glyat-B6J-VA
Gene Name
Product ID
S-CKO-00547
Gene Alias
A330009E03Rik; ACGNAT; CAT; GAT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Glyatem1flox/Cya mice (Catalog S-CKO-00547) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044976
NCBI RefSeq
NM_145935
Target Region
Exon 3~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
GLYAT, known as Glycine-N-acyltransferase, is an enzyme that catalyzes the transfer of acyl groups from acyl CoA to glycine, resulting in acyl glycine and coenzyme A. It is part of the phase II glycine conjugation pathway in the liver, which is crucial for maintaining adequate levels of free coenzyme A (CoASH) and detoxifying excess acyl-CoAs, such as benzoyl-CoA [3,4].
GLYAT has been shown to be lowly expressed in several cancers including liver cancer, clear cell renal cell carcinoma (ccRCC), and breast cancer. In liver cancer and ccRCC, overexpression of GLYAT inhibits cell proliferation and migration, and in vivo assays in nude mice confirm its tumor-suppressor function. It downregulates Rho-associated coiled-coil-containing protein kinase 1 (ROCK1), thus suppressing cancer progression [1]. In breast cancer, knockdown of GLYAT enhances cell proliferation and migration likely through activation of the PI3K/AKT/Snail pathway, which induces epithelial-mesenchymal transition (EMT) [2].
In conclusion, GLYAT plays a significant role in metabolic processes, especially in the glycine conjugation pathway for detoxification. Its downregulation is associated with tumor progression in multiple cancers, suggesting its potential as a prognostic biomarker and therapeutic target. The functional studies using in vivo models like nude mice have been instrumental in revealing its role in cancer-related biological processes [1,2].
References:
1. Xia, Yechen, Huang, Wentao, Jin, Guang-Zhi. 2024. GLYAT suppresses liver cancer and clear cell renal cell carcinoma progression by downregulating ROCK1 expression. In Translational cancer research, 13, 5097-5111. doi:10.21037/tcr-24-1412. https://pubmed.ncbi.nlm.nih.gov/39430840/
2. Tian, Xin, Wu, Lina, Jiang, Min, Liu, Caigang, Gao, Song. 2021. Downregulation of GLYAT Facilitates Tumor Growth and Metastasis and Poor Clinical Outcomes Through the PI3K/AKT/Snail Pathway in Human Breast Cancer. In Frontiers in oncology, 11, 641399. doi:10.3389/fonc.2021.641399. https://pubmed.ncbi.nlm.nih.gov/33968740/
3. Kühn, Stefan, Williams, Monray E, Dercksen, Marli, Sass, Jörn Oliver, van der Sluis, Rencia. 2023. The glycine N-acyltransferases, GLYAT and GLYATL1, contribute to the detoxification of isovaleryl-CoA - an in-silico and in vitro validation. In Computational and structural biotechnology journal, 21, 1236-1248. doi:10.1016/j.csbj.2023.01.041. https://pubmed.ncbi.nlm.nih.gov/36817957/
4. Badenhorst, Christoffel Petrus Stephanus, van der Sluis, Rencia, Erasmus, Elardus, van Dijk, Alberdina Aike. 2013. Glycine conjugation: importance in metabolism, the role of glycine N-acyltransferase, and factors that influence interindividual variation. In Expert opinion on drug metabolism & toxicology, 9, 1139-53. doi:10.1517/17425255.2013.796929. https://pubmed.ncbi.nlm.nih.gov/23650932/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen