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C57BL/6JCya-Ffar4em1flox/Cya
Common Name:
Ffar4-flox
Product ID:
S-CKO-00551
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ffar4-flox
Strain ID
CKOCMP-107221-Ffar4-B6J-VA
Gene Name
Ffar4
Product ID
S-CKO-00551
Gene Alias
Ffa4; GT01; Gpr120; Gpr129; KPG_013; O3far1; Pgr4
Background
C57BL/6JCya
NCBI ID
107221
Modification
Conditional knockout
Chromosome
19
Phenotype
MGI:2147577
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ffar4em1flox/Cya mice (Catalog S-CKO-00551) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000067098
NCBI RefSeq
NM_181748
Target Region
Exon 1
Size of Effective Region
~2.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ffar4, also known as G protein-coupled receptor 120, is a long-chain fatty acid receptor belonging to the rhodopsin-like G protein-coupled receptor (GPCR) family. It plays a crucial role in maintaining energy homeostasis by regulating adipogenesis, insulin sensitivity, and inflammation. It is involved in pathways such as Gq subunit-mediated CaMKKβ/AMPK signaling, and its activation helps maintain metabolic homeostasis [2,4,6,7].

Systemic and conditional knockout mouse models have provided valuable insights into Ffar4's function. In acute kidney injury (AKI) mouse models, knockout of Ffar4 aggravated renal function and pathological damage, while activation by TUG-891 alleviated disease severity. Ffar4 was found to regulate cellular senescence in injured kidneys and tubular epithelial cells via the AMPK/SirT3 signaling pathway [1]. In metabolic syndrome (MetS) mouse models, conventional and microglial conditional knockout of Ffar4 exacerbated high-fat diet-induced cognitive dysfunction and anxiety, while microglial overexpression improved these conditions, with Ffar4 regulating microglial activation through type I interferon signaling [3]. In heart failure with preserved ejection fraction secondary to metabolic syndrome (HFpEF-MetS) mouse models, systemic deletion of Ffar4 worsened diastolic function and microvascular rarefaction in male mice, and altered the balance of inflammatory oxylipins, increasing the pro-inflammatory state [5].

In conclusion, Ffar4 is essential for maintaining metabolic and immune homeostasis. The use of Ffar4 knockout and conditional knockout mouse models has revealed its significance in diseases such as AKI, MetS-related cognitive impairment, and HFpEF-MetS. These findings suggest that Ffar4 could be a potential therapeutic target for treating these and other related diseases.

References:
1. Yang, Letian, Wang, Bo, Guo, Fan, Fu, Ping, Ma, Liang. 2022. FFAR4 improves the senescence of tubular epithelial cells by AMPK/SirT3 signaling in acute kidney injury. In Signal transduction and targeted therapy, 7, 384. doi:10.1038/s41392-022-01254-x. https://pubmed.ncbi.nlm.nih.gov/36450712/
2. Stuttgen, Gage M, Sahoo, Daisy. . FFAR4: A New Player in Cardiometabolic Disease? In Endocrinology, 162, . doi:10.1210/endocr/bqab111. https://pubmed.ncbi.nlm.nih.gov/34043793/
3. Wang, Wei, Li, Jinyou, Cui, Siyuan, Chen, Yong Q, Zhu, Shenglong. 2024. Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome. In Science advances, 10, eadj7813. doi:10.1126/sciadv.adj7813. https://pubmed.ncbi.nlm.nih.gov/38306420/
4. Kiepura, Anna, Stachyra, Kamila, Olszanecki, Rafał. 2021. Anti-Atherosclerotic Potential of Free Fatty Acid Receptor 4 (FFAR4). In Biomedicines, 9, . doi:10.3390/biomedicines9050467. https://pubmed.ncbi.nlm.nih.gov/33923318/
5. Zhang, Naixin, Harsch, Brian, Zhang, Michael J, Murphy, Katherine A, O'Connell, Timothy D. 2023. FFAR4 regulates cardiac oxylipin balance to promote inflammation resolution in HFpEF secondary to metabolic syndrome. In Journal of lipid research, 64, 100374. doi:10.1016/j.jlr.2023.100374. https://pubmed.ncbi.nlm.nih.gov/37075982/
6. Oh, Da Young, Walenta, Evelyn. 2014. Omega-3 Fatty Acids and FFAR4. In Frontiers in endocrinology, 5, 115. doi:10.3389/fendo.2014.00115. https://pubmed.ncbi.nlm.nih.gov/25076939/
7. Kimura, Ikuo, Ichimura, Atsuhiko, Ohue-Kitano, Ryuji, Igarashi, Miki. 2019. Free Fatty Acid Receptors in Health and Disease. In Physiological reviews, 100, 171-210. doi:10.1152/physrev.00041.2018. https://pubmed.ncbi.nlm.nih.gov/31487233/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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