C57BL/6JCya-Acacaem1flox/Cya
Common Name:
Acaca-flox
Product ID:
S-CKO-00571
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Acaca-flox
Strain ID
CKOCMP-107476-Acaca-B6J-VB
Gene Name
Product ID
S-CKO-00571
Gene Alias
A530025K05Rik; Acac; Acc1; Gm738
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acacaem1flox/Cya mice (Catalog S-CKO-00571) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020843
NCBI RefSeq
XM_006531956
Target Region
Exon 5
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Acaca, also known as acetyl-CoA carboxylase 1, is a key enzyme controlling the rate of fatty acid synthesis. It plays a vital role in lipid metabolism and is involved in pathways such as the AMPK-PPARα-CPT1A axis, which is crucial for maintaining normal physiological functions related to lipid and mitochondrial metabolism [1].
In non-alcoholic fatty liver disease (NAFLD) research, a fatty liver mouse model was used. Inhibiting Acaca in vitro reduced intracellular lipid accumulation, improved mitochondrial dysfunction, and alleviated oxidative stress. This was achieved by mediating the AMPK-PPARα-CPT1A pathway [1].
In prostate cancer, ND630 can control Acaca and lipid reprogramming by regulating the expression of circKIF18B_003, suggesting a potential new target for treatment [2].
In patients with Acaca-related acetyl-CoA carboxylase deficiency, pathogenic variants in Acaca can lead to hypotonia, motor and intellectual developmental delay, and in one case, seizure [3].
In conclusion, Acaca is essential for lipid metabolism. Gene-knockout or related functional studies in mouse models have revealed its role in diseases like NAFLD and prostate cancer. Understanding Acaca's function provides insights into the mechanisms of these diseases and may offer new therapeutic directions.
References：
1. Dong, Jian, Li, Muzi, Peng, Runsheng, Qiao, Zilin, Sun, Na. 2024. ACACA reduces lipid accumulation through dual regulation of lipid metabolism and mitochondrial function via AMPK- PPARα- CPT1A axis. In Journal of translational medicine, 22, 196. doi:10.1186/s12967-024-04942-0. https://pubmed.ncbi.nlm.nih.gov/38395901/
2. Wu, Yu-Peng, Zheng, Wen-Cai, Huang, Qi, Xue, Xue-Yi, Xu, Ning. 2023. ND630 controls ACACA and lipid reprogramming in prostate cancer by regulating the expression of circKIF18B_003. In Journal of translational medicine, 21, 877. doi:10.1186/s12967-023-04760-w. https://pubmed.ncbi.nlm.nih.gov/38049827/
3. Shafieipour, Negin, Jafari Khamirani, Hossein, Kamal, Neda, Dianatpour, Mehdi, Dastgheib, Seyed Alireza. 2023. The third patient of ACACA-related acetyl-CoA carboxylase deficiency with seizure and literature review. In European journal of medical genetics, 66, 104707. doi:10.1016/j.ejmg.2023.104707. https://pubmed.ncbi.nlm.nih.gov/36709796/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen