C57BL/6JCya-Snrpd2em1flox/Cya
Common Name:
Snrpd2-flox
Product ID:
S-CKO-00593
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Snrpd2-flox
Strain ID
CKOCMP-107686-Snrpd2-B6J-VA
Gene Name
Product ID
S-CKO-00593
Gene Alias
1810009A06Rik; SMD2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snrpd2em1flox/Cya mice (Catalog S-CKO-00593) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049294
NCBI RefSeq
NM_026943.1
Target Region
Exon 2~3
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Snrpd2, a core component of the spliceosome, plays a crucial role in RNA splicing, a dynamic process regulated by the spliceosome in response to environmental stimuli. RNA splicing is essential for the maturation of pre-mRNA into functional mRNA, thereby affecting gene expression and various biological processes [1,2,3,4,6,7].
In the context of diseases, in hepatocellular carcinoma (HCC), Snrpd2 is the most highly upregulated Sm protein and acts as an oncogene. It modulates DDX39A intron retention with HNRNPL, leading to the expression of the DDX39A short variant which in turn mediates MYC mRNA nuclear export, forming a positive feedback loop [2]. In colorectal cancer, upregulated Snrpd2 can interact with the glutamic-proline (EP) domain of PABPN1, disrupt its liquid-liquid phase separation, and promote alternative polyadenylation of CTNNBIP1, driving cell proliferation and migration [3]. In melanoma, Snrpd2 is identified as a hub gene associated with metastasis and prognosis, and is also associated with immunotherapy [5]. Moreover, in the study of myotonic dystrophy type 1 (DM1), modest knockdown of Snrpd2 in DM1 fibroblasts and myoblasts significantly reduces DMPK expression and partially rescues MBNL-regulated alternative splicing dysfunction, revealing an unappreciated role for MBNL:spliceosomal protein stoichiometry in modulating the spliceopathy [4].
In conclusion, Snrpd2 is essential for RNA splicing and has significant implications in multiple disease areas. Through various functional studies, its role in cancer metastasis, oncogenic signaling in HCC, alternative polyadenylation in colorectal cancer, and spliceopathy in DM1 has been revealed. These findings from different disease-related research models contribute to a better understanding of the biological functions of Snrpd2 and offer potential targets for disease treatment [2,3,4,5].
References:
1. Bullones-Bolaños, Andrea, Araujo-Garrido, Juan Luis, Fernández-García, Jesús, Bernal-Bayard, Joaquín, Ramos-Morales, Francisco. 2022. SNRPD2 Is a Novel Substrate for the Ubiquitin Ligase Activity of the Salmonella Type III Secretion Effector SlrP. In Biology, 11, . doi:10.3390/biology11101517. https://pubmed.ncbi.nlm.nih.gov/36290420/
2. Chang, Cunjie, Li, Lina, Su, Ling, Chabot, Benoit, Chen, Jianxiang. 2024. Intron Retention of DDX39A Driven by SNRPD2 is a Crucial Splicing Axis for Oncogenic MYC/Spliceosome Program in Hepatocellular Carcinoma. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2403387. doi:10.1002/advs.202403387. https://pubmed.ncbi.nlm.nih.gov/39018261/
3. Hu, Zhijie, Li, Mengxia, Chen, Yufeng, Fu, Yonggui, Xu, Anlong. 2024. Disruption of PABPN1 phase separation by SNRPD2 drives colorectal cancer cell proliferation and migration through promoting alternative polyadenylation of CTNNBIP1. In Science China. Life sciences, 67, 1212-1225. doi:10.1007/s11427-023-2495-x. https://pubmed.ncbi.nlm.nih.gov/38811444/
4. Louis, Jiss M, Frias, Jesus A, Schroader, Jacob H, Berglund, J Andrew, Reddy, Kaalak. . Expression levels of core spliceosomal proteins modulate the MBNL-mediated spliceopathy in DM1. In Human molecular genetics, 33, 1873-1886. doi:10.1093/hmg/ddae125. https://pubmed.ncbi.nlm.nih.gov/39180495/
5. Chen, Ying, Wang, Dan, Li, Qingyun, Chen, Qiong, Chen, Yang. 2022. Identification of Three Genes Associated with Metastasis in Melanoma and Construction of a Predictive Model: A Multiracial Identification. In Journal of oncology, 2022, 4567063. doi:10.1155/2022/4567063. https://pubmed.ncbi.nlm.nih.gov/35637857/
6. Zhao, Ya-Dan, Yang, Cai-Xia, Du, Zhi-Qiang. 2023. Integrated single cell transcriptome sequencing analysis reveals species-specific genes and molecular pathways for pig spermiogenesis. In Reproduction in domestic animals = Zuchthygiene, 58, 1745-1755. doi:10.1111/rda.14493. https://pubmed.ncbi.nlm.nih.gov/37874861/
7. Wyatt, Christopher D R, Pernaute, Barbara, Gohr, André, Bonnal, Sophie, Irimia, Manuel. 2022. A developmentally programmed splicing failure contributes to DNA damage response attenuation during mammalian zygotic genome activation. In Science advances, 8, eabn4935. doi:10.1126/sciadv.abn4935. https://pubmed.ncbi.nlm.nih.gov/35417229/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen