C57BL/6JCya-Scamp1em1flox/Cya
Common Name:
Scamp1-flox
Product ID:
S-CKO-00603
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Scamp1-flox
Strain ID
CKOCMP-107767-Scamp1-B6J-VA
Gene Name
Product ID
S-CKO-00603
Gene Alias
4930505M11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Scamp1em1flox/Cya mice (Catalog S-CKO-00603) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022197
NCBI RefSeq
NM_029153
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Scamp1, Secretory Carrier-associated Membrane Protein 1, is the most widely expressed member of the SCAMP family. It is involved in endocytosis, with its NPF repeats in the N-terminal region binding to EH domain proteins like intersectin 1 and gamma-synergin, potentially mediating the budding of transport vesicles from the plasma membrane and trans-Golgi complex [4]. It may participate in endocytosis by recruiting clathrin coats to these membranes.
In cancer research, Scamp1 knockdown has shown significant impacts. In gastric cancer, it drives hyper-proliferation, as its knockdown suppresses GC cell proliferation in vitro and in vivo, and attenuates the Akt/MAPK/Stat signaling pathway [1]. In pancreatic β-cells, Scamp1 knockdown leads to insulin hypersecretion, and expression of a non-palmitoylated Scamp1 mutant in APT1-deficient cells rescues insulin hypersecretion and apoptosis [2]. In osteosarcoma, lncRNA Scamp1 promotes cell viability and invasion via the miR-26a-5p/ZEB2 axis [3]. In pediatric renal cell carcinoma, lncRNA Scamp1 promotes tumorigenesis under oxidative stress through miR-429 and its downstream targets ZEB1 and JUN [5]. In breast cancer, silencing Scamp1-TV2 inhibits cell proliferation, migration, and invasion, and promotes apoptosis by interacting with PUM2 to facilitate INSM1 mRNA degradation [6]. In glioma, knockdown of lncRNA Scamp1 suppresses malignant behaviors via the miR-499a-5p/LMX1A/NLRC5 pathway [7]. In ovarian cancer, lncRNA Scamp1 promotes cell invasion and angiogenesis through the miR-137/CXCL12 axis [8].
In conclusion, Scamp1 is crucial for endocytosis. Its role in various cancers, as revealed through loss-of-function experiments, indicates its potential as a therapeutic target. Understanding Scamp1's function in different disease conditions can help develop new strategies for cancer treatment and management.
References:
1. Ma, Gang, Yang, Yang, Cai, Fenglin, Ke, Bin, Deng, Jingyu. 2024. SCAMP1 silencing inhibits proliferation by attenuating multiple pro-survival signaling pathways in gastric cancer. In Journal of Cancer, 15, 5762-5772. doi:10.7150/jca.99610. https://pubmed.ncbi.nlm.nih.gov/39308691/
2. Dong, Guifang, Adak, Sangeeta, Spyropoulos, George, Wei, Xiaochao, Semenkovich, Clay F. 2023. Palmitoylation couples insulin hypersecretion with β cell failure in diabetes. In Cell metabolism, 35, 332-344.e7. doi:10.1016/j.cmet.2022.12.012. https://pubmed.ncbi.nlm.nih.gov/36634673/
3. Li, Rong, Chen, Zhen, Zhou, Yubo, Zhou, Peng, Ding, Lu. 2022. LncRNA SCAMP1 disrupts the balance between miR-26a-5p and ZEB2 to promote osteosarcoma cell viability and invasion. In Frontiers in oncology, 12, 967000. doi:10.3389/fonc.2022.967000. https://pubmed.ncbi.nlm.nih.gov/35992869/
4. Fernández-Chacón, R, Achiriloaie, M, Janz, R, Albanesi, J P, Südhof, T C. . SCAMP1 function in endocytosis. In The Journal of biological chemistry, 275, 12752-6. doi:. https://pubmed.ncbi.nlm.nih.gov/10777571/
5. Shao, Qiguo, Wang, Qi, Wang, Jianmin. 2019. LncRNA SCAMP1 regulates ZEB1/JUN and autophagy to promote pediatric renal cell carcinoma under oxidative stress via miR-429. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 120, 109460. doi:10.1016/j.biopha.2019.109460. https://pubmed.ncbi.nlm.nih.gov/31550675/
6. Tao, Wei, Ma, Jun, Zheng, Jian, Chen, Jiajia, Xue, Yixue. 2020. Silencing SCAMP1-TV2 Inhibited the Malignant Biological Behaviors of Breast Cancer Cells by Interaction With PUM2 to Facilitate INSM1 mRNA Degradation. In Frontiers in oncology, 10, 613. doi:10.3389/fonc.2020.00613. https://pubmed.ncbi.nlm.nih.gov/32670859/
7. Zong, Zheqi, Song, Yichen, Xue, Yixue, Li, Zhen, Liu, Yunhui. 2019. Knockdown of LncRNA SCAMP1 suppressed malignant biological behaviours of glioma cells via modulating miR-499a-5p/LMX1A/NLRC5 pathway. In Journal of cellular and molecular medicine, 23, 5048-5062. doi:10.1111/jcmm.14362. https://pubmed.ncbi.nlm.nih.gov/31207033/
8. Song, Ran, Liu, Zhihui, Lu, Lijuan, Liu, Fenglin, Zhang, Bei. 2020. Long Noncoding RNA SCAMP1 Targets miR-137/CXCL12 Axis to Boost Cell Invasion and Angiogenesis in Ovarian Cancer. In DNA and cell biology, 39, 1041-1050. doi:10.1089/dna.2019.5312. https://pubmed.ncbi.nlm.nih.gov/32401536/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen