C57BL/6NCya-Shmt2em1flox/Cya
Common Name:
Shmt2-flox
Product ID:
S-CKO-00633
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Shmt2-flox
Strain ID
CKOCMP-108037-Shmt2-B6N-VA
Gene Name
Product ID
S-CKO-00633
Gene Alias
2700043D08Rik; SHMT
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Shmt2em1flox/Cya mice (Catalog S-CKO-00633) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026470
NCBI RefSeq
NM_028230
Target Region
Exon 3~12
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Shmt2, or serine hydroxymethyltransferase 2, is a key enzyme in one-carbon metabolism. It uses pyridoxal-5'-phosphate (PLP) as a cofactor and can convert serine into glycine and a tetrahydrofolate-bound one-carbon unit, ultimately supporting thymidine and purine synthesis [2,3]. This process is crucial for amino acid and nucleotide metabolism, and is associated with multiple biological processes and disease conditions.
In disease-related studies, hepatocyte-specific ablation of Shmt2 in mice (KO model) showed that its absence increased serine and glycine levels in circulation, decreased liver methylation potential, and increased susceptibility to fatty liver disease. However, when fed a high-fat, fructose, and cholesterol diet, these mice had less inflammation and fibrosis, highlighting its stage-specific functions in non-alcoholic fatty liver disease (NAFLD) pathogenesis [4]. In Burkitt lymphoma, inhibition of Shmt2 (by knockdown or pharmacological compounds) led to anti-lymphoma effects both in vitro and in vivo. It reduced intracellular glycine and formate levels, inhibited the mTOR pathway, and triggered autophagic degradation of the oncogenic transcription factor TCF3, collapsing tonic B-cell receptor (BCR) signaling essential for lymphoma cell survival [1].
In conclusion, Shmt2 is essential for one-carbon metabolism, playing a role in nucleotide and amino acid synthesis. Through gene knockout models in mice, its functions in diseases like NAFLD and Burkitt lymphoma have been revealed. These studies help us understand its role in disease mechanisms, potentially providing new targets for therapeutic intervention.
References:
1. Wilke, Anne C, Doebele, Carmen, Zindel, Alena, Zenz, Thorsten, Oellerich, Thomas. . SHMT2 inhibition disrupts the TCF3 transcriptional survival program in Burkitt lymphoma. In Blood, 139, 538-553. doi:10.1182/blood.2021012081. https://pubmed.ncbi.nlm.nih.gov/34624079/
2. Zeng, Yuanyuan, Zhang, Jie, Xu, Mengmeng, Chen, Nannan, Chin, Y Eugene. 2021. Roles of Mitochondrial Serine Hydroxymethyltransferase 2 (SHMT2) in Human Carcinogenesis. In Journal of Cancer, 12, 5888-5894. doi:10.7150/jca.60170. https://pubmed.ncbi.nlm.nih.gov/34476002/
3. Walden, Miriam, Tian, Lei, Ross, Rebecca L, Greenberg, Roger A, Zeqiraj, Elton. 2019. Metabolic control of BRISC-SHMT2 assembly regulates immune signalling. In Nature, 570, 194-199. doi:10.1038/s41586-019-1232-1. https://pubmed.ncbi.nlm.nih.gov/31142841/
4. Chen, Guohua, Zhou, Guoli, Zhai, Lidong, Mottillo, Emilio, Wang, Jian. 2024. SHMT2 reduces fatty liver but is necessary for liver inflammation and fibrosis in mice. In Communications biology, 7, 173. doi:10.1038/s42003-024-05861-y. https://pubmed.ncbi.nlm.nih.gov/38347107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen